Shh and ROCK1 modulate the dynamic epithelial morphogenesis in circumvallate papilla development
Shh and ROCK1 modulate the dynamic epithelial morphogenesis in circumvallate papilla development
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DOI：
10.1016/j.ydbio.2008.10.034
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Jung, Han-Sung
中科院分区：
文献类型：
--
作者：
Kim, Jae-Young;Lee, Min-Jung;Jung, Han-Sung
在啮齿类动物中，在早期舌头发育过程中，周乳头（CVP）随着上皮形态发生的动态变化而发育。 CVP 发育的分子和细胞研究表明，在心尖和沟壁形成区域存在两种不同的机制，具有 Wnt11 和 Shh 的特定表达模式。使用过表达Shh、重要信号分子和各种抑制剂的体外器官培养物检查了分子相互作用，揭示了Wnt11和Shh表达在CVP形成中存在两种显着不同的机制。 Wnt 是一种众所周知的启动味觉乳头的关键分子，它将控制 CVP 顶端上皮的 Rho 激活和细胞骨架形成。相反，Shh 调节细胞增殖以分化味蕾并使上皮内陷以促进冯·埃布纳腺 (VEG) 的发育。基于这些结果，我们认为 Wnt11 和 Shh 的这些不同的分子信号级联在小鼠早期胚胎发育过程中 CVP 的特定形态发生和模式形成中发挥着至关重要的作用。 (C) 2008 Elsevier Inc. 保留所有权利。
In rodents, a circumvallate papilla (CVP) develops with dynamic changes in epithelial morphogenesis during early tongue development. Molecular and cellular studies of CVP development revealed that there would be two different mechanisms in the apex and the trench wall forming regions with specific expression patterns of Wnt11 and Shh. Molecular interactions were examined using in vitro organ culture with over-expression of Shh, important signalling molecules and various inhibitors revealed that there are two significant different mechanisms in CVP formation by Wnt11 and Shh expressions. Wnt, a well known key molecule to initiate taste papillae, would govern Rho activation and cytoskeleton formation in the apex epithelium of CVP. In contrast, Shh regulates the cell proliferation to differentiate taste buds and to invaginate the epithelium for development of von Ebner's gland (VEG). Based on these results, we suggest that these different molecular signalling cascades of Wnt11 and Shh would play crucial roles in specific morphogenesis and pattern formation of CVP during early mouse embryo development. (C) 2008 Elsevier Inc. All rights reserved.