EFFECT OF LACTOBACILLUS-RHAMNOSUS STRAIN GG (ATCC-53103) ON THE GROWTH OF STREPTOCOCCUS-SOBRINUS IN-VITRO
EFFECT OF LACTOBACILLUS-RHAMNOSUS STRAIN GG (ATCC-53103) ON THE GROWTH OF STREPTOCOCCUS-SOBRINUS IN-VITRO
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DOI：
10.1111/j.1600-0722.1995.tb00169.x
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发表时间：
1995-08-01
影响因子：
1.9
通讯作者：
SALMINEN, S
中科院分区：
文献类型：
--
作者：
MEURMAN, JH;ANTILA, H;SALMINEN, S
乳杆菌 GG 是一种最近鉴定的鼠李糖乳杆菌 GG 菌株 (ATCC 53103)，已被证明对多种细菌物种（包括链球菌）具有抑制活性。我们分离并研究了乳杆菌GG的抑制物质对某些口腔链球菌的作用。分离的物质的抑制活性较弱，但与未处理的对照相比，用该物质预处理的远缘链球菌观察到一些生长抑制。琼脂平板上的生长抑制区仅在 pH 值低于 5 时才明显，表明抑制活性仅限于较低的 pH 范围。生长曲线实验显示，有和没有分离物质的系列之间具有统计学上显着的抑制作用（P < 0.05）。当用抑制物质处理时，S. sobrinus 的超微结构没有受到影响。 GG乳杆菌本身不发酵蔗糖。这些结果为未来的研究提供了有趣的视角，重点关注正常菌群的保护功能，并尝试用危害较小的细菌替代口腔微生物群中的有害细菌。
Lactobacillus GG, a recently characterized L. rhamnosus GG strain (ATCC 53103), has been shown to exert inhibitory activity against a variety of bacterial species, including streptococci. We isolated and studied the effect of the inhibitory substance of Lactobacillus GG on some oral streptococci. The inhibitory activity of the isolated substance was weak, but some growth inhibition was observed in Streptococcus sobrinus pretreated with the substance in comparison with untreated controls. Zones of growth inhibition on agar plates were apparent only at pH values below 5, indicating that the inhibitory activity was restricted to a low pH range. Growth curve experiments showed a statistically significant inhibition between series with and without the isolated substance (P < 0.05). The ultrastructure of S. sobrinus was not affected when treated with the inhibitory substance. The Lactobacillus GG itself did not ferment sucrose. The results offer interesting perspectives for future research focusing on the protective function of normal flora and in the attempt to replace harmful bacterial species in oral microflora with less harmful ones.