The hypersensitivity to colonic distension of IBS patients can be transferred to rats through their fecal microbiota
The hypersensitivity to colonic distension of IBS patients can be transferred to rats through their fecal microbiota
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DOI：
10.1111/nmo.12103
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发表时间：
2013-04-01
影响因子：
3.5
通讯作者：
Bernalier-Donadille, A.
中科院分区：
文献类型：
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作者：
Crouzet, L.;Gaultier, E.;Bernalier-Donadille, A.
背景肠易激综合征(IBS)的两个特征是肠道微生物区系的改变和对结肠扩张的超敏反应。然而，肠道微生物区系在IBS患者内脏高敏感性中的作用还远未确定。我们研究的目的是确定肠道微生物区系是否与IBS的内脏高敏感性有关。方法观察诺和生大鼠对大肠扩张的疼痛反应和结肠粘膜参数。将IBS患者(IBS HMA大鼠)或非过敏健康志愿者(健康HMA大鼠)的粪便微生物群接种无菌(GF)大鼠。常规大鼠作为正常敏感度对照。使用培养和分子方法对人类和HMA大鼠的粪便样本进行了粪便微生物分析。关键结果：在诺氏杆菌感染的大鼠中，IBS肠道微生物区系(硫酸盐还原菌和肠杆菌科细菌较多，双歧杆菌较少)的微生物生态失调得以维持。IBS HMA大鼠因结直肠扩张而引起的腹部收缩次数显著高于健康HMA大鼠。健康HMA大鼠与常规大鼠之间无明显差异。三组大鼠的结肠顺应性、上皮细胞旁通透性和结肠粘膜肥大细胞密度相似。结论&我们在这里的推论表明，IBS患者对结肠扩张的敏感性可以通过粪便微生物群传递给大鼠。与微生物区系转移相关的粘膜改变与这种超敏反应无关。IBS微生物区系的改变可能通过特定的细菌代谢产物在IBS患者的超敏反应特征中发挥重要作用。
Background Alterations of intestinal microbiota and hypersensitivity to colonic distension are two features of the irritable bowel syndrome (IBS). However, the role of intestinal microbiota in visceral hypersensitivity of IBS patients is far to be established. The aim of our study was to determine whether the intestinal microbiota is involved in the visceral hypersensitivity in IBS. Methods The painful response to colorectal distension and colonic mucosal parameters were assessed in gnotobiotic rats. Germfree (GF) rats were inoculated with the fecal microbiota from IBS patients characterized by hypersensitivity to colorectal distension (IBS HMA rats) or from non-hypersensitive healthy volunteers (Healthy HMA rats). Conventional rats were studied as normosensitivity control. Fecal microbial analyses were carried out in human and HMA rats fecal samples using cultural and molecular approaches. Key Results The microbial dysbiosis of the IBS gut microbiota (more sulfate-reducing bacteria and Enterobacteriaceae and less bifidobacteria) could be maintained in gnotobiotic rats. The number of abdominal contractions in response to colorectal distensions was significantly higher in IBS HMA rats than in healthy HMA rats. No difference was observed between healthy HMA and conventional rats. Colorectal compliance, epithelial paracellular permeability, and density of colonic mucosal mast cells were similar in the three groups of rats. Conclusions & Inferences We herein showed that sensitivity to colonic distension of IBS patients can be transferred to rats by the fecal microbiota. Mucosal alterations associated with microbiota transfer are not involved in this hypersensitivity. The altered IBS microbiota may have important role in the hypersensitivity characterizing IBS patients through specific bacterial metabolites.