Impaired dendritic cell maturation in response to pandemic H1N109 influenza virus
Impaired dendritic cell maturation in response to pandemic H1N109 influenza virus
复制标题
DOI：
10.1016/j.jcv.2012.11.009
复制
发表时间：
2013-03-01
影响因子：
8.8
通讯作者：
Torresi, Joseph
中科院分区：
文献类型：
--
作者：
Chin, Ruth;Earnest-Silviera, Linda;Torresi, Joseph
背景：甲型h1n1流感大流行病毒感染导致预计不会因季节性流感感染而出现严重疾病风险的患者住院。目的：我们试图确定(i)严重大流行性流感感染患者的DC成熟是否受到损害，（ii）大流行性流感病毒诱导DC成熟的能力与季节性流感病毒不同，以及（iii）是否存在相关的无法激活记忆B细胞或诱导抗体的情况。研究设计：从确诊的急性甲型h1n1流感大流行感染者或健康接种疫苗的对照组中采集外周血单核细胞。将dc与PBMC区分开来，并测试它们在大流行病毒、季节性H3N2流感病毒或LPS刺激下成熟的能力。患者的血清样本用于评估血清向流感的转化，并测定PBMC中流感特异性记忆B细胞的水平。结果：当暴露于甲型H1N1/2009流感大流行病毒时，所有个体的dc均表现出可忽略不计的成熟标志物上调，但对季节性H3N2病毒和LPS表现出强烈反应。在两组和血清转化为病毒的患者中均获得了高水平的记忆B细胞。结论：总体而言，患者的DC在不同刺激下成熟的能力与对照组的DC没有差异。重要的是，与季节性病毒相比，panH1N109病毒未能在任何个体中诱导实质性的DC成熟，但这并未导致无法建立对病毒的记忆B细胞和抗体反应。(C) 2012年Elsevier b.v.出版。
Background: Infection with pandemic A/H1N1/2009 influenza virus led to hospitalisation of patients not expected to be at risk of severe disease from seasonal influenza infection.Objectives: We sought to establish whether (i) DC maturation was compromised in patients experiencing severe pandemic influenza infection, (ii) the pandemic virus differed from seasonal influenza virus in its ability to induce DC maturation and (iii) there was an associated inability to activate memory B cells or induce antibody.Study design: Peripheral blood mononuclear (PBMCs) cells were sampled from individuals with confirmed acute pandemic A/H1N1/2009 influenza infection or from healthy vaccinated controls. DCs were differentiated from the PBMC and tested for their ability to mature following stimulation with pandemic virus, seasonal H3N2 influenza virus or LPS. Serum samples from the patients were used to assess seroconversion to influenza and the levels of influenza specific memory B cells in PBMC were also determined.Results: DCs obtained from all individuals exhibited negligible maturation marker upregulation when exposed to pandemic A/H1N1/2009 virus but showed a strong response to the seasonal H3N2 virus and LPS. Robust levels of memory B cell were obtained in both groups and patients seroconverted to the virus.Conclusions: Overall, the ability of patient's DC to mature in response to different stimuli was no different to that of control subjects DCs. Importantly, panH1N109 virus failed to induce substantial DC maturation in any individual, contrasting with seasonal virus, but this did not result in failure to mount memory B cell and antibody responses to the virus. (C) 2012 Published by Elsevier B. V.