Patch-clamp measurements of elementary chloride currents activated by the putative inhibitory transmitter GABA and glycine in mammalian spinal neurons.
Patch-clamp measurements of elementary chloride currents activated by the putative inhibitory transmitter GABA and glycine in mammalian spinal neurons.
复制标题
膜片钳测量哺乳动物脊髓神经元中假定的抑制性递质 GABA 和甘氨酸激活的元素氯电流。
DOI：
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发表时间：
1983
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影响因子：
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通讯作者：
Jochen Bormann
中科院分区：
文献类型：
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作者：
B. Sakmann;O. Hamill;Jochen Bormann
本文报道了用膜片钳技术测定培养的脊髓神经元索马膜全细胞和单一Cl-电流的方法。为了将由两种假定的抑制性递质GABA和甘氨酸激活的单通道Cl-电流与其他膜电流分开，使用等渗KCl移液管溶液测量“细胞附着”膜贴片。为了分离“无细胞”膜贴片中的单一Cl-电流，在移液器和浴中均使用对称的等渗Tris Cl-溶液。在两个激动剂分子与其各自的受体结合后，单个Cl-通道被打开。根据del Castillo和Katz（1957）以及Katz和Thesleff（1957）最初提出的激活和脱敏机制，描述了单个受体通道复合物的门控行为。
Methods for measuring whole-cell and unitary Cl- currents in the soma membrane of cultured spinal cord neurons by the patch-clamp technique are described. To separate single channel Cl- currents activated by the two putative inhibitory transmitters GABA and glycine from other membrane currents, isotonic KCl pipette solutions are used for measurements on "cell-attached" membrane patches. To isolate unitary Cl- currents in "cell-free" membrane patches, symmetrical, isotonic Tris Cl- solutions are used in both the pipette and the bath. Single Cl- channels are opened following binding of two agonist molecules to their respective receptors. The gating behavior of single receptor-channel complexes is described in terms of the activation and desensitization mechanism suggested originally by del Castillo and Katz (1957) and Katz and Thesleff (1957).