Surface Plasmon Coupling Effect of Gold Nanoparticles with Different Shape and Size on Conventional Surface Plasmon Resonance Signal
Surface Plasmon Coupling Effect of Gold Nanoparticles with Different Shape and Size on Conventional Surface Plasmon Resonance Signal
复制标题
不同形状和尺寸的金纳米粒子的表面等离子体耦合对常规表面等离子体共振信号的影响
DOI：
10.1007/s11468-010-9141-z
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发表时间：
2010-09-01
期刊：
影响因子：
3
通讯作者：
Zhang, Aidong
中科院分区：
文献类型：
--
作者：
Mustafa, Damra E.;Yang, Tianming;Zhang, Aidong
传统表面等离子体共振（SPR）信号增强的金纳米粒子标记策略已被广泛用于小分子与其相互作用伙伴结合的敏感测定。然而，不同尺寸和形状的金纳米颗粒对SPR信号的影响尚不清楚。本文制备了不同尺寸的纳米棒、纳米球和纳米八面体三种金纳米颗粒，研究了它们在固定激发波长670 nm下对常规SPR信号的影响。研究发现，在固定浓度下，不同形状和尺寸的金纳米颗粒由于其等离子体吸光度带的不同，导致SPR信号（即共振角移）发生变化。对于不同纵向吸光带的金纳米棒，分别将金纳米棒悬浮液引入到SPR传感器芯片表面时，可以清晰地观察到三个常规SPR信号区域。一个区域是与SPR激发波长重合或接近的纵向吸光度带，抑制了SPR角移。第二个区域是624 ~ 639 nm和728 ~ 763 nm的纵向吸光度带，SPR共振角位移适度增加。在700 ~ 726 nm的纵向吸光度波段发现了第三个区域，导致SPR角移响应显著增加。这一现象可以通过计算SPR角移响应与金纳米棒纵向吸光度带的相关性来解释。对于纳米球和纳米八面体，SPR角移响应与颗粒形状和尺寸有关，且随纳米颗粒尺寸的增加而持续增加。因此，本文提出了一种选择金纳米粒子作为标签的准则，用于测定与固定相互作用伙伴结合的小分子的SPR。
The labeling strategy with gold nanoparticles for the conventional surface plasmon resonance (SPR) signal enhancement has been frequently used for the sensitive determination of small molecules binding to its interaction partners. However, the influence of gold nanoparticles with different size and shape on SPR signal is not known. In this paper, three kinds of gold nanoparticles, namely nanorods, nanospheres, and nanooctahedrons with different size, were prepared and used to investigate their effects on the conventional SPR signal at a fixed excitation wavelength 670 nm. It was found that the SPR signal (i.e., resonant angle shift) was varied with the shapes and sizes of gold nanoparticles in suspension at a fixed concentration due to their different plasmon absorbance bands. For gold nanorods with different longitudinal absorbance bands, three conventional SPR signal regions could be clearly observed when the gold nanorod suspensions were separately introduced onto the SPR sensor chip surface. One region was the longitudinal absorbance bands coinciding with or close to the SPR excitation wavelength that suppressed the SPR angle shift. The second region was the longitudinal absorbance bands at 624 to 639 and 728 to 763 nm that produced a moderate increase on the SPR resonant angle shift. The third region was found for the longitudinal absorbance bands from 700 to 726 nm that resulted in a remarkable increase in the SPR angle shift responses. This phenomenon can be explained on the basis of calculation of the correlation of SPR angle shift response with the gold nanorod longitudinal absorbance bands. For nanospheres and nanooctahedrons, the SPR angle shift responses were found to be particle shape and size dependent in a simple way with a sustaining increase when the sizes of the nanoparticles were increased. Consequently, a guideline for choosing gold nanoparticles as tags is suggested for the SPR determination of small molecules with binding to the immobilized interaction partners.