Hippocampal neurons respond uniquely to topographies of various sizes and shapes.
Hippocampal neurons respond uniquely to topographies of various sizes and shapes.
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DOI：
10.1088/1758-5082/2/3/035005
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发表时间：
2010-09
期刊：
影响因子：
9
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Fozdar DY;Lee JY;Schmidt CE;Chen S
为了开发用于神经工程应用的接口，许多研究已经调查了神经元在微制造拓扑上的行为。然而，很少有研究同时探索具有不同特征尺寸和形状的形貌对轴突生长和极化的影响。因此，在这里，我们研究了线阵列的影响，（脊槽）和微米（~2 μm）和纳米（~300 nm）尺寸的孔，在石英（SiO2）中图案化，对（1）粘附，（2）轴突建立（极化），（3）轴突长度，（4）轴突排列和（5）细胞形态，研究神经元对特征尺寸和几何形状的反应。使用光学和扫描电子显微镜分析神经元。的地形被发现有一个可以忽略不计的影响，细胞附着，但引起轴突极化的显着增加，更频繁地发生在亚微尺度的功能比微尺度的功能。观察到神经元形成较长的轴突线比孔和光滑的表面上，轴突排列平行或垂直的线功能。细胞形态分析表明，表面特征影响索马、轴突和生长锥的形态。结果表明，在生物材料表面引入微尺度和亚微尺度形貌可以增强生物材料调节神经发育和再生的能力。
A number of studies have investigated the behavior of neurons on microfabricated topography for the purpose of developing interfaces for use in neural engineering applications. However, there have been few studies simultaneously exploring the effects of topographies having various feature sizes and shapes on axon growth and polarization in the first 24 h. Accordingly, here we investigated the effects of arrays of lines (ridge grooves) and holes of microscale (~2 μm) and nanoscale (~300 nm) dimensions, patterned in quartz (SiO2), on the (1) adhesion, (2) axon establishment (polarization), (3) axon length, (4) axon alignment and (5) cell morphology of rat embryonic hippocampal neurons, to study the response of the neurons to feature dimension and geometry. Neurons were analyzed using optical and scanning electron microscopy. The topographies were found to have a negligible effect on cell attachment but to cause a marked increase in axon polarization, occurring more frequently on sub-microscale features than on microscale features. Neurons were observed to form longer axons on lines than on holes and smooth surfaces; axons were either aligned parallel or perpendicular to the line features. An analysis of cell morphology indicated that the surface features impacted the morphologies of the soma, axon and growth cone. The results suggest that incorporating microscale and sub-microscale topographies on biomaterial surfaces may enhance the biomaterials’ ability to modulate nerve development and regeneration.