The effects of purified eicosapentaenoic and docosahexaenoic acids on arterial thrombosis tendency and platelet function in rats
The effects of purified eicosapentaenoic and docosahexaenoic acids on arterial thrombosis tendency and platelet function in rats
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DOI：
10.1016/s0005-2760(97)00184-7
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发表时间：
1998-02-23
期刊：
影响因子：
--
通讯作者：
Hornstra, G
中科院分区：
文献类型：
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作者：
Nieuwenhuys, CMA;Hornstra, G
使用经过充分验证的大鼠模型，比较了膳食二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对体内动脉血栓形成的影响。还测定了血小板聚集（由水蛭素化全血中的胶原或二磷酸腺苷触发）、血栓素形成（TxB（2））和血小板磷脂脂肪酸组成。喂食含氢化椰子油或向日葵籽油饲料的动物分别作为促血栓形成和抗血栓形成对照。在第一项研究中，大鼠被喂食EPA和DHA乙酯（MIX）的混合物，其量逐渐增加，结果表明4%的n-3脂肪酸对血栓形成倾向具有最佳的降低效果。饮食中给予MIX进一步导致剂量依赖性地促进胶原诱导聚集后的解聚，这与血小板TxB（2）形成的减少显著相关。在随后的比较研究中，EPA和DHA乙酯对血栓形成倾向、血小板聚集和TxB（2）形成的影响程度相似。此外，两种多烯均增加血小板对血栓素A（2）的表观敏感性，这与动脉血栓形成倾向呈负相关。我们的结论是，EPA和DHA在大鼠体内对动脉血栓形成有类似的减少作用，并在体外对选定的血小板功能有相当的影响。(C)1998年Elsevier Science B.V.
Dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were compared for their effects on arterial thrombus formation in vivo using a well validated rat model. Platelet aggregation (triggered by collagen or adenosine diphosphate in whole hirudinized blood), thromboxane formation (TxB(2)) and platelet phospholipid fatty acid composition were measured also. Animals fed diets containing hydrogenated coconut oil or sunflower seed oil served as prothrombotic and antithrombotic controls, respectively. In a first study, rats were fed a mixture of EPA and DHA ethyl esters (MIX) in increasing amounts and results indicated that 4% of n-3 fatty acids had an optimum reducing effect on thrombosis tendency. Dietary administration of MIX further resulted in a dose-dependent promotion of disaggregation after collagen-induced aggregation, which significantly correlated with the reduction in platelet TxB(2) formation. In a subsequent comparative study, both EPA and DHA ethyl esters affected thrombosis tendency, platelet aggregation and TxB(2) formation to a similar extent. In addition, both polyenes increased the apparent thromboxane A(2)-sensitivity of platelets, which appeared negatively related to arterial thrombosis tendency. We conclude that EPA and DHA have similar reducing effects on arterial thrombogenesis in vivo in rats and have comparable effects on the selected platelet functions in vitro. (C) 1998 Elsevier Science B.V.