Ribosomal and Mitochondrial DNA Analyses of Xiphinema americanum-Group Populations.

Ribosomal and Mitochondrial DNA Analyses of Xiphinema americanum-Group Populations.
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DOI:
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发表时间:
2006-12
影响因子:
1.3
通讯作者:
S. Lazarova;G. Malloch;Cláudio Oliveira;J. Hübschen;R. Neilson
S. Lazarova;G. Malloch;Cláudio Oliveira;J. Hübschen;R. Neilson
中科院分区:
生物学4区
文献类型:
--
作者:
S. Lazarova;G. Malloch;Cláudio Oliveira;J. Hübschen;R. Neilson

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对来自不同地理位置的24个美洲西菲纳种群的18S核糖体DNA (rDNA)和线粒体DNA (mtDNA)的细胞色素氧化酶I区进行了测序。序列进行系统发育分析和比较。18S rDNA强烈表明,葡萄牙的X. pachtaicum、X. fax(两个居群)和X. americanum s.l.居群与其他20个居群存在差异,而mtDNA表明居群之间存在一定的异质性。在系统发育上,基于mtDNA,北美洲和亚洲、南美洲和大洋洲的美洲狮种群存在明显的二分法。18S rDNA和mtDNA序列的分析支持了美洲狮类群的经典分类问题,并对美洲狮类群内的物种形成程度提出了质疑。
The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanum-group populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.