The carrier effect in the secondary response to hapten‐protein conjugates. V. Use of antilymphocyte serum to deplete animals of helper cells
The carrier effect in the secondary response to hapten‐protein conjugates. V. Use of antilymphocyte serum to deplete animals of helper cells
复制标题
对半抗原蛋白缀合物的二次反应中的载体效应 V. 使用抗淋巴细胞血清来消耗动物的辅助细胞。
DOI：
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发表时间：
1971
影响因子：
5.4
通讯作者：
N. Mitchison
中科院分区：
文献类型：
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作者：
D. Mosier;L. W. P. Coppleson;Nat;Acad;S. SciU.;N. London;N. Mitchison
用兔抗小鼠淋巴细胞血清（ALS）处理牛血清白蛋白、鸡γ球蛋白或钥孔血蓝蛋白致敏的小鼠，可抑制对这些抗原的继发性应答，尤其是过继性继发性应答。然而，如果转移的细胞用蛋白质的NIP（4-羟基-5-碘-3-硝基-苯乙酰基-）或DNP（2，4-二硝基苯基-）缀合物加强，则它们产生抗蛋白应答的能力可以通过添加针对半抗原的细胞而部分恢复。这样的半抗原引发的细胞可以例如通过用与另一种载体缀合的半抗原免疫来获得。因此，这些辅助细胞可以被视为半抗原特异性的。
Treatment of mice primed with bovine serum albumin, chicken γ‐globulin or keyhole limpet hemocyanin with rabbit anti‐mouse lymphocyte serum (ALS) depresses the secondary response, and particularly the adoptive secondary response, to these antigens. If, however, the transferred cells are boosted with NIP (4‐hydroxy‐5‐iodo‐3‐nitro‐phenacetyl‐) or DNP (2,4‐dinitrophenyl‐) conjugates of the protein with which they had been primed, their ability to make an anti‐protein response can be partially restored by adding cells primed against the hapten. Such hapten‐primed cells can be obtained, for example, by immunization with the hapten conjugated to another carrier. These helper cells can therefore be regarded as hapten‐specific.