Adherence of type 1-fimbriated Escherichia coli to uroepithelial cells -: More in diabetic women than in control subjects
Adherence of type 1-fimbriated Escherichia coli to uroepithelial cells -: More in diabetic women than in control subjects
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DOI：
10.2337/diacare.25.8.1405
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发表时间：
2002-08-01
期刊：
影响因子：
16.2
通讯作者：
Hoepelman, AIM
中科院分区：
文献类型：
--
作者：
Geerlings, SE;Meiland, R;Hoepelman, AIM
目的：女性糖尿病患者比非糖尿病患者更容易出现菌尿。由于大肠杆菌对复发性尿路感染（UTIs）的非糖尿病患者阴道细胞的粘附性优于健康对照者，因此假设大肠杆菌对复发性尿路感染（UTIs）的非糖尿病患者阴道细胞的粘附性优于健康对照者阴道细胞。大肠杆菌更多地粘附在糖尿病妇女的泌尿上皮细胞上，或者是因为尿液中排泄的物质（例如，白蛋白、葡萄糖和TammHorsfall蛋白）或由于泌尿上皮细胞的差异。研究设计和方法-将25名糖尿病妇女和19名对照受试者的T24膀胱细胞系和泌尿上皮细胞与三种不同的E.结果：1型菌毛大肠杆菌平均数为1.25;大肠杆菌粘附糖尿病细胞和对照细胞的粘附率分别为12.9和6.1（P = 0.001），而P-菌毛型大肠杆菌粘附糖尿病细胞和对照细胞的粘附率分别为12.9和6.1（P = 0.001）。大肠埃希氏菌为8.8和8.1（P = 0.8），无菌毛大肠埃希氏菌为8.8和8.1（P = 0.8）。大肠埃希氏菌分别为2.7和3.4（P = 0.4）。各种物质的添加均不影响E.结论：1型菌毛大肠杆菌与T24膀胱癌细胞株的细胞毒作用不明显。大肠杆菌对糖尿病患者尿上皮细胞的粘附性高于对照组。
OBJECTIVE - Women with diabetes have bacteriuria more often than women without diabetes. Because Escherichia coli adhere better to vaginal cells of nondiabetic patients with recurrent urinary tract infections (UTIs) than to those obtained from healthy control subjects, it was hypothesized that E. coli adhere more to the uroepithelial cells of diabetic women, either because of substances excreted in the urine (e.g., albumin, glucose, and Tamm Horsfall protein) or because of a difference in the uroepithelial cells.RESEARCH DESIGN AND METHODS- A T24 bladder cell line and uroepithelial cells of 25 diabetic women and 19 control subjects were incubated with three different E. coli strains.RESULTS - The mean numbers of type 1-fimbriated E. coli that adhered to diabetic and control cells were 12.9 and 6.1 (P = 0.001), respectively, whereas those of P-fimbriated E. coli were 8.8 and 8.1 (P = 0.8), and those of nonfimbriated E. coli were 2.7 and 3.4 (P = 0.4). The addition of various substances did not influence the adherence of E. coli to a T24 bladder cell line.CONCLUSIONS - Type 1-fimbriated E. coli adhere more to diabetic than to control uroepithelial cells.