Genome shuffling of Streptomyces gilvosporeus for improving natamycin production.
Genome shuffling of Streptomyces gilvosporeus for improving natamycin production.
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DOI：
10.1021/jf300663w
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发表时间：
2012-05
影响因子：
6.1
通讯作者：
Jianmei Luo;Jianshu Li;D. Liu;Feng Liu;Yanting Wang;Xin-Ran Song;Min Wang
中科院分区：
文献类型：
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作者：
Jianmei Luo;Jianshu Li;D. Liu;Feng Liu;Yanting Wang;Xin-Ran Song;Min Wang
通过基因组改组形式的递归原生质体融合来进行褐黄孢链霉菌ATCC 13326的纳他霉素生产的改进。经过4轮基因组改组，筛选出遗传稳定性最好的菌株GS 4-21，摇瓶培养96 h，纳他霉素产量达到4.69 ± 0.05 g/L，比出发菌株pop-72 A（r）07和ATCC 13326分别提高了97.1%和379%。与出发菌株ATCC 13326相比，重组菌株GS 4-21具有更高的多态性. 54个蛋白质在重组GS 4-21和初始菌株ATCC 13326之间显示出差异表达水平。在这些蛋白质中，34个蛋白质被上调，20个蛋白质被下调。在上调的蛋白质中，一种蛋白质，葡萄糖激酶调节蛋白，参与纳他霉素的生物合成。这一综合分析将为了解纳他霉素在S.黄孢子菌。
Improvement of natamycin production by Streptomyces gilvosporeus ATCC 13326 was performed by recursive protoplast fusion in a genome-shuffling format. After four rounds of genome shuffling, the best producer, GS 4-21, with genetic stability was obtained and its production of natamycin reached 4.69 ± 0.05 g/L in shaking flask after 96 h cultivation, which was increased by 97.1% and 379% in comparison with the highest parental strain pop-72A(r)07 and the initial strain ATCC 13326, respectively. Compared with the initial strain ATCC 13326, the recombinant GS 4-21 presented higher polymorphism. Fifty-four proteins showed differential expression levels between the recombinant GS 4-21 and initial strain ATCC 13326. Of these proteins, 34 proteins were upregulated and 20 proteins were downregulated. Of the upregulated proteins, one protein, glucokinase regulatory protein, was involved in natamycin biosynthesis. This comprehensive analysis would provide useful information for understanding the natamycin metabolic pathway in S. gilvosporeus.