Prevention of lethal experimental infection of C57BL/6 mice by vaccination with Brucella abortus strain RB51 expressing Neospora caninum antigens
Prevention of lethal experimental infection of C57BL/6 mice by vaccination with Brucella abortus strain RB51 expressing Neospora caninum antigens
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DOI：
10.1016/j.ijpara.2007.04.020
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发表时间：
2007-11-01
影响因子：
4
通讯作者：
Sriranganathan, Nammalwar
中科院分区：
文献类型：
--
作者：
Ramamoorthy, Sheela;Sanakkayala, Neelima;Sriranganathan, Nammalwar
由细胞内原生动物寄生虫犬新孢子虫（Neospora caninum）引起的牛流产是全世界养牛业的主要关注点。强的Th 1免疫应答是针对N.犬的牛种布氏杆菌菌株RB 51目前用作牛种布氏杆菌病减毒活疫苗。菌株RB 51也可用作异源蛋白表达的表达载体。在本研究中，N。犬MIC 1、MIC 3、GRA 2、GRA 6和SRS 2在B中单独表达。流产杆菌菌株RB 51每种重组RB 51菌株诱导N.在C57 BL/6小鼠中评估犬特异性免疫。通过两次腹膜内接种（间隔4周）免疫小鼠。第二次免疫后5周，在体外用N.犬全细胞裂解物抗原。N.在接种小鼠的血清中检测到IgG 1和IgG 2a亚型的犬特异性抗体。接种组和对照组的小鼠用2 × 107 N进行攻毒。犬速殖子i. p.，接种后观察28天。所有未接种疫苗的对照小鼠均在7天内死亡。MIC 1和GRA 6疫苗组中的小鼠得到完全保护，而SRS 2、GRA 2和MIC 3疫苗组中的小鼠得到部分保护，并经历10-50%的死亡率。非重组RB 51载体对照组经历了69%的平均保护。这些结果表明，N. B中的犬。牛种布氏杆菌菌株RB 51是一种开发抗布鲁氏菌病和新孢子虫病多价疫苗的新方法。(c)2007年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Bovine abortions caused by the intracellular protozoal parasite Neospora caninum are a major concern to cattle industries worldwide. A strong Th1 immune response is required for protection against N. caninum. Brucella abortus strain RB51 is currently used as a live, attenuated vaccine against bovine brucellosis. Strain RB51 can also be used as an expression vector for heterologous protein expression. In this study, putative protective antigens of N. caninum MIC1, MIC3, GRA2, GRA6 and SRS2, were expressed individually in B. abortus strain RB51 The ability of each of the recombinant RB51 strains to induce N. caninum-specific immunity was assessed in C57BL/6 mice. Mice were immunised by two i.p. inoculations, 4 weeks apart. Five weeks after the second immunisation, spleen cells from the vaccinated mice secreted high levels of IFN-gamma and IL-10 upon in vitro stimulation with N. caninum whole cell lysate antigens. N. caninum-specific antibodies of both IgG1 and IgG2a subtypes were detected in the serum of the vaccinated mice. Mice in the vaccinated and control groups were challenged with 2 X 107 N. caninum tachyzoites i.p. and observed for 28 days after vaccination. All unvaccinated control mice died within 7 days. Mice in the MIC1 and GRA6 vaccine groups were completely protected while the mice in the SRS2, GRA2 and MIC3 vaccinated groups were partially protected and experienced 10-50% mortality. The non-recombinant RB51 vector control group experienced an average protection of 69%. These results suggest that expression of protective antigens of N. caninum in B. abortus strain RB51 is a novel approach towards the development of a multivalent vaccine against brucellosis and neosporosis. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.