Clustering of antigenic sites recognized by cytotoxic T lymphocyte clones in the amino terminal half of SV40 T antigen.
Clustering of antigenic sites recognized by cytotoxic T lymphocyte clones in the amino terminal half of SV40 T antigen.
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SV40 T 抗原氨基末端一半的细胞毒性 T 淋巴细胞克隆识别的抗原位点聚集。
DOI：
10.1016/0042-6822(88)90483-7
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Tevethia,SS
中科院分区：
文献类型：
--
作者：
Tanaka,Y;Tevethia,MJ;Kalderon,D;Smith,AE;Tevethia,SS
用细胞毒性T淋巴细胞（CTL）克隆研究了SV 40 T抗原氨基端半区CTL识别抗原位点的分布。用B6/pSV 3 T320 GV细胞免疫C57 BL/6（B6）小鼠脾细胞，再用表达SV 40 T109 -708氨基酸的B6/pPVU-5- 70 K细胞进行体外刺激，然后克隆。建立的所有10个CTL克隆的识别序列定位在SV 40 T抗原的氨基末端一半的氨基酸109和271之间。这10个CTL克隆的精细作图定义了三个不同的抗原位点。这三个位点分别通过缺失SV 40 T抗原氨基酸193-211、220-223和220-228而消除。从用B6-K/S11-S24细胞免疫的136只小鼠的脾细胞建立另外的CTL克隆，所述B6-K/S11-S24细胞合成缺失氨基酸127-250的SV 40 T抗原。这些CTL克隆均不与B6/pSV 3 T3 - 20 GV细胞发生反应。这些CTL克隆识别定位在SV 40 T抗原的羧基末端一半的抗原位点。我们的研究结果表明，在SV 40 T抗原的氨基末端的一半的抗原位点是紧密聚集在氨基酸193和271之间，最有可能在193和228之间。
The distribution of antigenic sites recognized by cytotoxic T lymphocytes (CTL) in the amino terminal half of SV40 T antigen was studied using SV40-specific CTL clones. Spleen cells of C57BL/6 (B6) mice immunized with B6/pSV3T320GV cells, which synthesize a truncated SV40 T antigen of amino acids 1–368, were restimulatedin vitrowith B6/pPVU-5-70K cells expressing SV40 T antigen of amino acids 109–708 and then cloned. The recognition sequence for all 10 CTL clones established mapped in the amino terminal half of SV40 T antigen between amino acids 109 and 271. Fine mapping of these 10 CTL clones defined three distinct antigenic sites. These three sites were abolished by the deletion of SV40 T antigen amino acids 193–211, 220–223, and 220–228, respectively. Additional CTL clones were established from spleen cells of 136 mice immunized with B6-K/S11–S24 cells, which synthesize a SV40 T antigen missing amino acids 127–250. None of these CTL clones reacted with B6/pSV3T3-20GV cells. These CTL clones recognized an antigenic site(s) which mapped in the carboxy terminal half of SV40 T antigen. Our results indicate that the antigenic sites in the amino terminal half of SV40 T antigen are tightly clustered between amino acids 193 and 271 and most probably between 193 and 228.