THE SIR2 GENE FAMILY, CONSERVED FROM BACTERIA TO HUMANS, FUNCTIONS IN SILENCING, CELL-CYCLE PROGRESSION, AND CHROMOSOME STABILITY
THE SIR2 GENE FAMILY, CONSERVED FROM BACTERIA TO HUMANS, FUNCTIONS IN SILENCING, CELL-CYCLE PROGRESSION, AND CHROMOSOME STABILITY
复制标题
DOI：
10.1101/gad.9.23.2888
复制
发表时间：
1995-12-15
影响因子：
10.5
通讯作者：
BOEKE, JD
中科院分区：
文献类型：
--
作者：
BRACHMANN, CB;SHERMAN, JM;BOEKE, JD
基因组沉默是转录调控的一种基本机制，但对沉默的保守机制知之甚少。我们在此报道了四个酿酒酵母SIR2沉默基因（HSTs）的同源物的发现，以及该基因家族从细菌到哺乳动物的保守性。至少有三个HST基因在沉默中起作用；HST1过表达恢复了sir2突变体的转录沉默，而hst3和hst4双突变体在端粒沉默方面存在缺陷。此外，HST3和HST4共同参与适当的细胞周期进程、辐射抗性和基因组稳定性，在沉默与这些基本细胞过程之间建立了新的联系。
Genomic silencing is a fundamental mechanism of transcriptional regulation, yet little is known about conserved mechanisms of silencing. We report here the discovery of four Saccharomyces cerevisiae homologs of the SIR2 silencing gene (HSTs), as well as conservation of this gene family from bacteria to mammals. At least three HST genes can function in silencing; HST1 overexpression restores transcriptional silencing to a sir2 mutant and hst3 hst4 double mutants are defective in telomeric silencing. In addition, HST3 and HST4 together contribute to proper cell cycle progression, radiation resistance, and genomic stability, establishing new connections between silencing and these fundamental cellular processes.