Lipid Activation of Coagulation Factor III Apoprotein (Tissue Factor) - Reconstitution of the Protein-Membrane Complex
Lipid Activation of Coagulation Factor III Apoprotein (Tissue Factor) - Reconstitution of the Protein-Membrane Complex
复制标题
凝血因子 III 脱辅基蛋白（组织因子）的脂质激活 - 蛋白质膜复合物的重建
DOI：
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发表时间：
1980
影响因子：
6.7
通讯作者：
William K. Konigsberg
中科院分区：
文献类型：
--
作者：
S. Carson;William K. Konigsberg
Summary Coagulation factor III (tissue factor) is required for significant activation of factor X in the presence of factor VIIa. Factor III is a membrane protein which requires bound phospholipids for activity, and removal of lipids during purification of factor III abolishes its activity. The activity can be recovered if lipids are added to the apoprotein under conditions known to favor reconstitution of membrane proteins into phospholipid bilayers. Indeed, incorporation of factor III into vesicular phospholipids is the essential event for recovery of factor III function, and is promoted by cadmium chloride. Using factor III from human placenta, we have studied the interactions of cadmium, phospholipids, and apoprotein which result in successful protein membrane reconstitution. The effects of phospholipids and cadmium can be independently optimized. Cadmium promotes reconstitution of factor III either with preformed phospholipid vesicles or as the vesicles form during slow dialysis. The incorporation of apoprotein into preformed vesicles is completed within four minutes. These studies demonstrate successful activation of factor III apoprotein by incorporation into phospholipid vesicles and optimization of this activation using cadmium chloride.
