Identification of four mud crab species (genus Scylla) using ITS-1 and 16S rDNA markers
Identification of four mud crab species (genus Scylla) using ITS-1 and 16S rDNA markers
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DOI：
10.1051/alr:2004007
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发表时间：
2004-01-01
影响因子：
1.1
通讯作者：
Numachi, K
中科院分区：
文献类型：
--
作者：
Imai, H;Cheng, JH;Numachi, K
从4种青蟹成虫组织中提取基因组DNA，采用聚合酶链反应（PCR）技术扩增了青蟹核核糖体DNA（rDNA）和线粒体DNA（mtDNA）16 S rRNA的第一个转录间隔区（ITS-1）。和S. serrata，S. paramamosain与紫螯青蟹S.以橄榄科植物为模板。利用ITS-1区，发现产物片段长度的变化可用于区分S. serrata和S. olivacea与另外两个种的区别。另2种（S. paramamosain与紫螯青蟹S. tranquebarica）可以用限制性内切酶Hha I鉴定。使用16 S rDNA，通过用Dra 1和HindIII双酶切的PCR-限制性片段长度多态性（RFLP）鉴定所有4个物种。这些遗传标记可用于青蟹属的杂交育种研究和青蟹幼蟹的野外调查。
The first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA and mitochondrial DNA 16S rRNA were amplified by Polymerase chain reaction (PCR) using genomic DNA extracted from adult tissue of four species of Scylla spp. and the first zoeal stages of S. serrata, S. paramamosain and S. olivacea as template. Using the ITS-1 region, variation in product fragment length was found to be useful for distinguishing S. serrata and S. olivacea from two other species. The other two species (S. paramamosain and S. tranquebarica) could be identified using the restriction endonuclease Hha I. Using 16S rDNA, all four species were identified using PCR-restriction fragment length polymorphism (RFLP) by double digestion with Dra1 and HindIII. These genetic markers can be used for hybridization breeding studies and in field studies of larval and juvenile mud crabs of the genus Scylla.