Diagnostic role of microRNA expression profile in the serum of pregnant women with fetuses with neural tube defects
Diagnostic role of microRNA expression profile in the serum of pregnant women with fetuses with neural tube defects
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孕妇血清中microRNA表达谱对神经管缺陷胎儿的诊断作用
DOI：
10.1111/j.1471-4159.2012.07812.x
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发表时间：
2012-08-01
影响因子：
4.7
通讯作者：
Yuan, Zhengwei
中科院分区：
文献类型：
--
作者：
Gu, Hui;Li, Hui;Yuan, Zhengwei
J.神经化学。 (2012) 122, 641649. 摘要 母体血清中胎盘 microRNA (miRNA) 的发现为无创产前诊断开辟了新的可能性。然而，胎儿患有神经管缺陷 (NTD) 的孕妇血清中 miRNA 的表达尚未得到表征。在本文中，我们探索了血清 miRNA 作为 NTD 胎儿孕妇血清中潜在生物标志物。通过使用覆盖 887 个人类 miRNA 的 miRNA 微阵列，我们发现了 17 个 miRNA 在 NTD 胎儿孕妇和正常妊娠妇女血清中的表达发生显着变化。定量逆转录 PCR (qRT-PCR) 分析验证了 6 种 miRNA（miR-142-3p、miR-144、miR-720、miR-575、miR-765 和 miR-1182）的表达上调，而 miR-1275 的表达下调。为了确定这些 miRNA 是否与妊娠相关，我们比较了分娩前和分娩后母体血清样本中的 miRNA 水平。其中 6 个 miRNA 在产后血清中迅速减少 (p < 0.05)。此外，通过受试者工作特征（ROC）曲线分析，这六种miRNA组合的ROC曲线下面积（AUC）为0.803（p < 0.001）。因此，我们揭示了患有 NTD 胎儿的孕妇血清中六种与妊娠相关的 miRNA 失调，并强调了血清 miRNA 作为胎儿 NTD 诊断和预测生物标志物的临床潜力。
J. Neurochem. (2012) 122, 641649. Abstract The discovery of placental microRNAs (miRNAs) in maternal serum has opened up new possibilities for non-invasive prenatal diagnosis. However, the expression of miRNAs in the serum of pregnant women with fetuses with neural tube defects (NTDs) has not been characterized. In this article, we explored serum miRNAs as potential biomarkers in the serum of pregnant women with NTD fetuses. By using a miRNA microarray that covers 887 human miRNAs, we revealed 17 miRNAs with significant change in expression in serum of pregnant women with NTD fetuses and women with normal pregnancies. Quantitative reverse-transcription PCR (qRT-PCR) analysis validated that the expression for six miRNAs (miR-142-3p, miR-144, miR-720, miR-575, miR-765, and miR-1182) was up-regulated and that for miR-1275 was down-regulated. To determine whether these miRNAs were related to pregnancy, we compared the miRNA levels in pre- and post-delivery maternal serum samples. Six of these miRNAs were rapidly reduced in post-delivery serum (p < 0.05). Moreover, by receiver operating characteristic (ROC) curve analysis, the area under the ROC curve (AUC) of combining these six miRNAs was 0.803 (p < 0.001). Thus, we reveal six pregnancy-associated miRNAs that are deregulated in the serum of pregnant women with NTD fetuses and highlight the clinical potential of serum miRNAs as biomarkers for diagnosis and prognostication of fetal NTDs.