Effect of a hyaluronan synthase suppressor, 4-methylumbelliferone, on B16F-10 melanoma cell adhesion and locomotion
Effect of a hyaluronan synthase suppressor, 4-methylumbelliferone, on B16F-10 melanoma cell adhesion and locomotion
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DOI：
10.1016/j.bbrc.2004.07.041
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发表时间：
2004-09-03
影响因子：
3.1
通讯作者：
Takagaki, K
中科院分区：
文献类型：
--
作者：
Kudo, D;Kon, A;Takagaki, K
透明质酸（HA）是一种普遍存在的细胞外基质的主要成分。它参与细胞粘附和运动，从而参与肿瘤转移。我们以前报道过4- methylumbellliferone （MU）抑制HA的合成，可能是研究HA功能的有用工具。我们在这里证明了黑色素瘤细胞表面HA的形成及其释放到培养基中受到MU的抑制。粘附和运动实验显示，MU可剂量依赖性地抑制黑色素瘤细胞的粘附和运动。相反，外源性透明质酸处理增强了粘连和运动。因此，阻止细胞表面HA的形成降低了黑色素瘤细胞的粘附和运动，提示MU可能具有抑制肿瘤转移的作用。(C) 2004爱思唯尔公司版权所有。
Hyaluronan (HA) is a ubiquitous, major component of the extracellular matrix. It is involved in cell adhesion and locomotion, and hence in tumor metastasis. We have previously reported that 4-methylumbelliferone (MU) inhibits HA synthesis and may be a useful tool for examining the functions of HA. We here demonstrate that the formation of cell surface HA by melanoma cells and its release into the culture medium are inhibited by MU. Adhesion and locomotion assays revealed that the adhesion and locomotion of melanoma cells were dose-dependently inhibited by MU. Conversely, treatment with exogenous HA enhanced both adhesion and locomotion. Thus, preventing the formation of cell surface HA reduced both the adhesion and locomotion of melanoma cells, suggesting that MU may act as an inhibitor of tumor metastasis. (C) 2004 Elsevier Inc. All rights reserved.