Regulation of the Expression of the Human Preprogastrin‐Releasing Peptide Gene and Post‐translational Processing of Its Gene Product
Regulation of the Expression of the Human Preprogastrin‐Releasing Peptide Gene and Post‐translational Processing of Its Gene Product
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人前胃泌素释放肽基因表达调控及其基因产物的翻译后加工
DOI：
10.1111/j.1749-6632.1988.tb23873.x
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发表时间：
1988
影响因子：
5.2
通讯作者：
J. Way
中科院分区：
文献类型：
--
作者：
J. Battey;A. Lebacq‐Verheyden;G. Krystal;S. Markowitz;Oliver Sartor;J. Way
胃泌素释放肽（GRP）是一种27个氨基酸的神经肽和生长因子，与两栖动物的四肽bombesin同源。*2修饰的羧基末端七肽，在两种肽中是相同的，代表了与高亲和力细胞表面受体结合所必需和充分的生物活性区域。GRP存在于哺乳动物的大脑中，在那里它影响体温、饱腹感和许多其他中枢稳态功能！GRP也广泛存在于肠道周围的周围神经中，“在那里它刺激胃肠胰激素的释放”并调节胃肠运动。此外，GRP还存在于胎儿支气管周围上皮的神经内分泌细胞中，以及人肺和甲状腺的神经内分泌细胞肿瘤，即小细胞肺癌（SCLC）、肺类癌、甲状腺髓样癌中。在一定条件下，GRP刺激了一些培养的SCLC细胞系、lcI6正常人支气管上皮细胞和Swiss 3T3小鼠胚胎成纤维细胞系的生长。“在瑞士3T3细胞中，GRP与高亲和力的细胞表面受体结合，引发有丝分裂信号后，随后通过一系列复杂的第二信使进行转导，包括细胞内pH值升高、细胞内游离钙、磷脂酰肌醇代谢和蛋白激酶C活性的变化。”这些事件之后是c-myc和c-fos基因的短暂转录诱导，增加DNA合成和细胞分裂。在一些培养的人SCLC细胞系中，低至1 nM的GRP浓度可引起细胞内游离钙2'和磷脂酰肌醇转换的快速和短暂增加：2可被针对肽羧基末端的单克隆抗体（2a1l）阻断。该单克隆抗体特异性地损害了克隆的生长
The gastrin-releasing peptide (GRP) is a 27 amino acid neuropeptide and growth factor that is homologous to the amphibian tetradecapeptide bombesin.'*2 The amidated carboxyl-terminal heptapeptide, which is identical in the two peptides, represents the biologically active region' that is necessary and sufficient for binding to high-affinity cell surface receptors.' GRP is found in mammalian brain,5 where it influences body temperature, satiety, and many other central homeostatic functions! GRP is also widely represented in peripheral nerves surrounding the gut,' where it stimulates the release of a number of gastroenteropancreatic hormones' and modulates gastrointestinal motility.' In addition, GRP is found in fetal neuroendocrine cells of the peribronchial epithelium," as well as in neuroendocrine cell tumors from the human lung and thyroid, namely small cell lung cancer (SCLC)," pulmonary carcinoid tumor,'* and medullary carcinoma of the thyroid." Under certain circumstances, GRP stimulates the growth of some cultured SCLC cell lines,lcI6 normal human bronchial epithelial cells," and the Swiss 3T3 mouse embryo fibroblast cell line.'' In Swiss 3T3 cells, after the GRP mitogenic signal is initiated by binding of GRP to high-affinity cell surface receptors, it is subsequently transduced through a complex series of second messengers, including an elevation of intracellular pH and changes in intracellular free calcium, phosphatidylinositol metabolism, and protein kinase C activity." These events are followed by transient transcriptional induction of the c-myc and c-fos genes," increased DNA synthesis, and cell division. In some cultured human SCLC cell lines, concentrations of GRP as low as I nM elicit a rapid and transient increase in intracellular free calcium2' and phosphatidylinositol turnover:2 which can be blocked by a monoclonal antibody ( 2 A l l ) directed against the carboxyl terminus of the peptide. This same monoclonal antibody specifically imparied the clonal growth in
DOI：
10.1016/s0021-9258(18)32209-9
发表时间：
1983-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Westendorf;A. Schonbrunn
通讯作者：
J. Westendorf;A. Schonbrunn
影响因子：
56.9
作者：
BRIGGS, MR;KADONAGA, JT;TJIAN, R
通讯作者：
TJIAN, R
人胰岛素基因和 cDNA 在异源哺乳动物系统中的表达。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Laub,O;Rutter,WJ
通讯作者：
Rutter,WJ