Odd-chain fatty acids as a biomarker for dietary fiber intake: a novel pathway for endogenous production from propionate
Odd-chain fatty acids as a biomarker for dietary fiber intake: a novel pathway for endogenous production from propionate
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DOI：
10.3945/ajcn.117.152702
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发表时间：
2017-06-01
影响因子：
7.1
通讯作者：
Klaus, Susanne
中科院分区：
文献类型：
--
作者：
Weitkunat, Karolin;Schumann, Sara;Klaus, Susanne
背景：2型糖尿病的风险与血浆中奇链脂肪酸(OCFAs；十五烷酸（15:0）和十七烷酸（17:0）]，它们被认为是人类乳制品脂肪摄入量的生物标志物。然而，啮齿动物研究表明OCFAs是由肠道衍生的丙酸内源性合成的。丙酸盐随着膳食纤维的摄入而增加，并已被证明可以改善胰岛素敏感性。目的：我们假设膳食纤维通过一种新的内源性途径产生OCFAs。设计：在一项随机、双盲交叉研究中，16名健康个体分别补充纤维素（30 g/d）、菊粉（30 g/d）或丙酸盐（6 g/d） 7天。此外，用不同浓度的丙酸盐培养人肝癌细胞。采用气相色谱法测定血浆磷脂和肝癌细胞中的OCFAs含量。结果：纤维素对血浆OCFA水平没有影响，而菊粉和丙酸分别使五酸升高了17% （P < 0.05）和13% （P = 0.05）。对十七烷酸的影响更为明显，因为菊粉（11%,P < 0.01）和丙酸盐（13%,P < 0.001）几乎所有参与者的十七烷酸水平都有所提高。此外，细胞培养实验显示丙酸盐与OCFA水平呈正相关（R-2 = 0.99, P < 0.0001），而棕榈酸盐（R-2 = 0.83, P = 0.004）呈负相关（R-2 = 0.99, P < 0.0001）。结论：我们的数据显示，肠源性丙酸可用于人类OCFAs的肝脏合成。因此，OCFAs与2型糖尿病风险降低的关联可能也与膳食纤维的摄入有关，而不仅仅是乳制品脂肪。该试验注册在www.germanctr.deas DRKS00010121。
Background: The risk of type 2 diabetes is inversely correlated with plasma concentrations of odd-chain fatty acids [OCFAs; pentadecanoic acid (15: 0) and heptadecanoic acid (17: 0)], which are considered as biomarkers for dairy fat intake in humans. However, rodent studies suggest that OCFAs are synthesized endogenously from gut-derived propionate. Propionate increases with dietary fiber consumption and has been shown to improve insulin sensitivity.Objective: We hypothesized that OCFAs are produced in humans from dietary fibers by a novel endogenous pathway. Design: In a randomized, double-blind crossover study, 16 healthy individuals were supplemented with cellulose (30 g/d), inulin (30 g/d), or propionate (6 g/d) for 7 d. In addition, human hepatoma cells were incubated with different propionate concentrations. OCFAs were determined in plasma phospholipids and hepatoma cells by gas chromatography.Results: Cellulose did not affect plasma OCFA levels, whereas inulin and propionate increased pentadecanoic acid by similar to 17% (P < 0.05) and 13% (P = 0.05), respectively. The effect on heptadecanoic acid was even more pronounced, because it was elevated in almost all participants by inulin (11%; P < 0.01) and propionate (13%; P < 0.001). Furthermore, cell culture experiments showed a positive association between propionate and OCFA levels (R-2 = 0.99, P < 0.0001), whereas palmitate (16: 0) was negatively correlated (R-2 = 0.83, P = 0.004).Conclusions: Our data show that gut-derived propionate is used for the hepatic synthesis of OCFAs in humans. The association of OCFAs with a decreased risk of type 2 diabetes may therefore also relate to dietary fiber intake and not only dairy fat. This trial was registered at www.germanctr.deas DRKS00010121.