Apoptosis induced by ischemia-reperfusion and fasting in gastric mucosa compared to small intestinal mucosa in rats
Apoptosis induced by ischemia-reperfusion and fasting in gastric mucosa compared to small intestinal mucosa in rats
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DOI：
10.1023/a:1005695031233
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发表时间：
2001-03-01
影响因子：
3.1
通讯作者：
Fujimoto, K
中科院分区：
文献类型：
--
作者：
Fukuyama, K;Iwakiri, R;Fujimoto, K
研究了缺血再灌注和48小时禁食对大鼠胃黏膜细胞凋亡的影响，并与小肠黏膜进行了比较。氟烷麻醉下，大鼠腹腔动脉或肠系膜上动脉闭塞60 min后再灌注。腹腔动脉的阻塞减少了胃的血流量，肠系膜动脉的阻塞减少了小肠的血流量。另取大鼠禁食48小时，观察禁食对粘膜细胞凋亡的影响。缺血再灌注或禁食后检测碎片DNA与总DNA的比值、电泳和免疫组织化学染色。缺血再灌注后胃黏膜细胞凋亡不明显，而肠黏膜细胞凋亡明显增加。此外，禁食48小时后，小肠细胞凋亡被诱导，而胃细胞凋亡未被诱导。说明大鼠胃黏膜对缺血再灌注和禁食诱导的细胞凋亡不像小肠黏膜那样敏感。
The effect of ischemia-reperfusion and 48-hr fasting on apoptosis was characterized in rat gastric mucosa and compared to small intestinal mucosa. Under halothane anesthesia, the celiac artery or superior mesenteric artery in the rat was occluded for 60 min followed by reperfusion. Occlusion of the celiac artery reduced blood flow in the stomach and occlusion of the mesenteric artery reduced blood flow in the small intestine. Additional rats were fasted for 48 hr to evaluate the effect of fasting on mucosal apoptosis. The ratios of fragmented DNA to total DNA, electrophoresis, and immunohistochemical staining were examined after ischemia-reperfusion or fasting. Apoptosis was not induced significantly in the gastric mucosa after ischemia-reperfusion, although it increased dramatically in the intestinal mucosa after ischemia-reperfusion. Further, after 48 fasting, apoptosis was induced in the small intestine, but not in the stomach. These results indicate that rat gastric mucosa is not as sensitive as small intestinal mucosa to ischemia-reperfusion or fasting-induced apoptosis.