Split-GFP lamin as a tool for studying C. elegans LMN-1 dynamics in vivo.
Split-GFP lamin as a tool for studying C. elegans LMN-1 dynamics in vivo.
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DOI：
10.17912/micropub.biology.001022
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发表时间：
2023
影响因子：
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通讯作者：
Starr, Daniel A
中科院分区：
文献类型：
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作者：
Gregory, Ellen F;Ragle, James Matthew;Ward, Jordan D;Starr, Daniel A
我们设计了一种荧光融合蛋白C。通过将GFP的第十一条β链融合到内源性lmn-1基因座处的LMN-1的N-末端，将GFP的第十一条β链与LMN-1的N-末端融合。当与GFP 1 -10共表达时，在多种体细胞的核周边观察到GFP 11：：LMN-1。纯合子gfp 11：：lmn-1动物具有正常数量的存活胚胎。然而，gfp 11：：lmn-1的动物有轻微的游泳缺陷。虽然功能不完全，但GFP 11：：LMN-1菌株比其他已发表的荧光LMN-1系更健康，使其成为研究核纤层蛋白的有价值的试剂。
We engineered a fluorescent fusion protein of C. elegans lamin, by fusing the eleventh beta strand of GFP to the N-terminus of LMN-1 at the endogenous lmn-1 locus. When co-expressed with GFP1-10, GFP11::LMN-1 was observed at the nuclear periphery of a wide variety of somatic cells. Homozygous gfp11::lmn-1 animals had normal numbers of viable embryos. However, the gfp11::lmn-1 animals had a mild swimming defect. While not completely functional, the GFP11::LMN-1 strain is more healthy than other published fluorescent LMN-1 lines, making it a valuable reagent for studying lamins.