Rickettsia lusitaniae associated with Ornithodoros yumatensis (Acari: Argasidae) from two caves in Yucatan, Mexico
Rickettsia lusitaniae associated with Ornithodoros yumatensis (Acari: Argasidae) from two caves in Yucatan, Mexico
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DOI：
10.1016/j.ttbdis.2016.09.003
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发表时间：
2016-01-01
影响因子：
3.2
通讯作者：
Labruna, Marcelo B.
中科院分区：
文献类型：
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作者：
Sanchez-Montes, Sokani;Guzman-Cornejo, Carmen;Labruna, Marcelo B.
立克次体属包括专性细胞内细菌，由几种食血节肢动物如蜱、跳蚤和吸吮虱传播。特别是硬蜱（硬蜱科）被认为是墨西哥致病性立克次体的主要媒介。然而，与墨西哥软蜱有关的单一立克次体只有两种记录。在这项研究中，我们从墨西哥尤卡坦州的两个蝙蝠洞中寻找了13只成年和8只玉马泰鸟（Ornithodoros yumatensis）的寄生蜱（argasid ticks）的立克次体DNA。此外，在墨西哥恰帕斯州的一个洞穴中收集了一只与圆齿蛾有关的幼虫，以证实蜱的分类测定。其中，9只蜱（43%）产生了立克次体gltA基因的预期PCR产物。对这些PCR阳性蜱进行了针对立克次体gltA、ompA和ompB基因的附加PCR检测。这些基因的部分DNA序列显示，它们与来自葡萄牙的一种分离株卢西塔尼亚立克次体同源性为99-100%，与felis和hoogstraalii立克次体亲缘关系密切。根据本研究的结果，分布在墨西哥的立克次体从6种增加到7种。(C) 2016 Elsevier GmbH版权所有。
The genus Rickettsia includes obligate intracellular bacteria transmitted by several hematophagous arthropods such as ticks, fleas and sucking lice. In particular hard ticks (Ixodidae) have been cited as the main vectors of pathogenic rickettsiae in Mexico. However, there have been only two records of a single Rickettsia species associated with Mexican soft ticks (Argasidae). In this study, we searched for rickettsial DNA in argasid ticks (13 adults and eight nymphs of Ornithodoros yumatensis) from two bat caves in the state of Yucatan, Mexico. Additionally one larva collected in a cave from Chiapas, Mexico, and associated with Desmodus rotundus was used to corroborate the tick taxonomic determination. Of these, nine ticks (43%) yielded expected PCR products for the rickettsial gltA gene. These PCR-positive ticks were tested with additional PCR protocols targeting the rickettsial genes gltA, ompA and ompB. DNA partial sequences from these genes showed 99-100% identities with Rickettsia lusitaniae, an agent isolated from O. erraticus in Portugal, and closely related to R. felis and R. hoogstraalii. Based on the results from this study, the inventory of rickettsiae distributed in Mexico increases from six to seven species. (C) 2016 Elsevier GmbH. All rights reserved.