Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63
Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63
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DOI：
10.1128/cdli.12.11.1317-1321.2005
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发表时间：
2005-11-01
期刊：
影响因子：
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通讯作者：
Peiris, JSM
中科院分区：
文献类型：
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作者：
Chan, KH;Cheng, VCC;Peiris, JSM
采用20例严重急性呼吸综合征（SARS）冠状病毒（CoV）感染患者的系列血清，通过中和试验和亚型特异性免疫荧光（IF）试验，确定了SARS冠状病毒感染的血清学应答特征。SARS冠状病毒总免疫球蛋白（IG）（IgG、伊加和IgM [IgGAM]）是第一个可检测的抗体。存活患者（n = 14）和死亡患者（n = 6）之间的血清转换时间无差异。尽管11例患者中有8例在感染后7个月仍能通过IF试验检测到SARS CoV IgM，但几何平均滴度从感染后1个月的282降至7个月的19（P = 0.001）。相反，中和抗体和SARS CoV IgGAM和IgG抗体滴度在此期间保持稳定。SARS冠状病毒抗体应答有时与先前存在的人冠状病毒OC 43、229 E和NL 63的IF IgG抗体滴度增加有关。来自疱疹病毒的病毒衣壳抗原的IF IgG滴度没有变化，疱疹病毒用作不相关的对照，EB病毒。相比之下，OC43感染的患者，可能也有229E感染，没有事先暴露于SARS CoV，对感染病毒特异性抗体增加，但对SARS CoV没有。在解释IF血清学时，需要了解冠状病毒之间的交叉反应性抗体应答。
The serological response profile of severe acute respiratory syndrome (SARS) coronavirus (CoV) infection was defined by neutralization tests and subclass-specific immunofluorescent (IF) tests using serial sera from 20 patients. SARS CoV total inummoglobulin (Ig) (IgG, IgA, and IgM [IgGAM]) was the first antibody to be detectable. There was no difference in time to seroconversion between the patients who survived (n = 14) and those who died (n = 6). Although SARS CoV IgM was still detectable by IF tests with 8 of 11 patients at 7 months postinfection, the geometric mean titers dropped from 282 at I month postinfection to 19 at 7 months (P = 0.001). In contrast, neutralizing antibody and SARS CoV IgGAM and IgG antibody titers remained stable over this period. The SARS CoV antibody response was sometimes associated with an increase in preexisting IF IgG antibody titers for human coronaviruses OC43, 229E, and NL63. There was no change in IF IgG titer for virus capsid antigen from the herpesvirus that was used as an unrelated control, Epstein-Barr virus. In contrast, patients who had OC43 infections, and probably also 229E infections, without prior exposure to SARS CoV had increases of antibodies specific for the infecting virus but not for SARS CoV. There is a need for awareness of cross-reactive antibody responses between coronaviruses when interpreting IF serology.