Synthesis of atrial natriuretic peptides and studies on structural factors in tissue specificity.
Synthesis of atrial natriuretic peptides and studies on structural factors in tissue specificity.
复制标题
心房钠尿肽的合成及组织特异性结构因素的研究。
DOI：
10.1016/0006-291x(84)90418-2
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Sugiyama,M;Fukumi,H;Grammer,RT;Misono,KS;Yabe,Y;Morisawa,Y;Inagami,T
采用固相法合成了含有25个氨基酸残基的心房利钠肽ANF IV和含有21个氨基酸残基的心房利钠肽ANF V，并与K3Fe(CN)6氧化形成二硫桥。合成ANF IV的尿钠活性是合成ANF V的5070倍，而长肽段的鸡直肠平滑肌松弛活性仅是合成ANF V的2.5倍。两种肽均抑制去甲肾上腺素诱导的兔主动脉收缩。较短的肽ANF V的效率比较长的肽ANF IV低300倍。这表明，ANF IV的羧基末端似乎对肾脏和血管组织中的受体亲和力有调节作用。
Two atrial natriuretic peptides, containing 25 amino acid residues, ANF IV, and 21 amino acid residues, ANF V, were synthesized by a solid phase method and oxidized with K3Fe(CN)6to form a disulfide bridge. Synthetic ANF IV exhibited a natriuretic activity with an ED5070 times higher than that of synthetic ANF V, whereas the longer peptide was only 2.5 times more potent in chick rectal smooth muscle relaxant activity. Both peptides inhibited norepinephrine-induced contraction of rabbit aorta. The shorter peptide, ANF V, was 300 times less efficient than the longer peptide, ANF IV. It is proposed that the carboxy-terminal of ANF IV seems to have a modulating effect on receptor affinity in kidney and vascular tissue.