AP-1 regulates the expression of IL17-4 and IL17-5 in the pacific oyster Crassostrea gigas
AP-1 regulates the expression of IL17-4 and IL17-5 in the pacific oyster Crassostrea gigas
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AP-1 调节太平洋牡蛎 IL17-4 和 IL17-5 的表达
DOI：
10.1016/j.fsi.2019.12.080
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发表时间：
2020
影响因子：
4.7
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Wang Liyan;Sun Jiejie;Wu Zhaojun;Lian Xingye;Han Shuo;Huang Shu;Yang Chuanyan;Wang Lingling;Song Linsheng
激活蛋白-1（AP-1）在诱导免疫效应物产生以响应细胞应激和细菌感染中起重要作用。本研究从太平洋牡蛎（Crassostreagigas）中分离到一个AP-1（命名为CgAP-1），它由290个氨基酸组成，包括一个Jun结构域和一个碱性亮氨酸拉链（bZIP）结构域，与牡蛎ChAP-1的同源性为98.6%。hongkongensis，在系统发育树中归属于无脊椎动物的分支。CgAP-1基因mRNA在各组织中均有表达，在血细胞中表达量最高，在粒细胞中表达量最高。LPS刺激后6 h，血细胞中CgAP-1基因mRNA表达水平显著上调（是PBS组的8.53倍，p< 0.01），CgAP-1蛋白可转位至细胞核内。LPS刺激后24 h，CgAP-1-RNAi牡蛎血细胞中白细胞介素17 s（CgIL 17 - 4和CgIL 17 -5）的mRNA转录水平显著下降，分别是EGFP-RNAi牡蛎的0.37倍（p< 0.05）和0.17倍（p< 0.01）。结果表明，CgAP-1通过调控CgIL 17 s的表达，在牡蛎免疫应答中发挥重要作用。
The activator protein-1 (AP-1) plays an important role in inducing the immune effector production in response to cellular stress and bacterial infection. In the present study, an AP-1 was identified from Pacific oysterCrassostreagigas(designed asCgAP-1) and its function was investigated in response against lipopolysaccharide (LPS) stimulation.CgAP-1 was consisted of 290 amino acids including a Jun domain and a basic region leucine zipper (bZIP) domain.CgAP-1 shared 98.6% similarities withChAP-1 from oysterC. hongkongensis,and assigned into the branch of invertebrates in the phylogenetic tree. The mRNA transcripts ofCgAP-1gene were detected in all tested tissues with highest expression level in hemocytes, especially in granulocytes. The mRNA expression level ofCgAP-1gene in hemocytes was significantly up-regulated (8.53-fold of that in PBS group,p< 0.01) at 6 h after LPS stimulation.CgAP-1 protein could be translocated into the nucleus of oyster hemocytes after LPS stimulation. The mRNA transcripts of interleukin17s (CgIL17-4andCgIL17-5) in the hemocytes ofCgAP-1-RNAi oysters decreased significantly at 24 h after LPS stimulation, which were 0.37-fold (p< 0.05) and 0.17-fold (p< 0.01) compared with that inEGFP-RNAi oysters, respectively. The results suggested thatCgAP-1 played an important role in the immune response of oyster by regulating the expression ofCgIL17s.