THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE TOBACCO CHLOROPLAST GENOME - ITS GENE ORGANIZATION AND EXPRESSION
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE TOBACCO CHLOROPLAST GENOME - ITS GENE ORGANIZATION AND EXPRESSION
复制标题
DOI：
10.1002/j.1460-2075.1986.tb04464.x
复制
发表时间：
1986-09-01
期刊：
影响因子：
11.4
通讯作者：
SUGIURA, M
中科院分区：
文献类型：
--
作者：
SHINOZAKI, K;OHME, M;SUGIURA, M
烟草（Nicotiana tabacum var.亮黄4）叶绿体DNA的测定。它包含两个相同的25 339 bp的反向重复序列，这两个重复序列被86 684 bp和18 482 bp的单拷贝区隔开。已经定位了4种不同的rRNA，30种不同的tRNA，39种不同的蛋白质和11种其他预测的蛋白质编码基因。其中15个基因含有内含子。印迹杂交显示，所有的rRNA和tRNA基因和27个蛋白质基因，到目前为止分析的转录在叶绿体中，迄今为止检查的分裂基因的主要成绩单剪接。已经发现了5个编码蛋白质的序列，这些蛋白质与来自人类线粒体的还原链NADH脱氢酶的组分同源。如果“三取二”和“U：N摆动”机制在叶绿体中起作用，则从它们的基因预测的30个tRNA足以读取所有密码子。在酵母中自主复制的两个序列也被绘制出来。序列分析和表达分析表明叶绿体基因具有原核和真核的特征。
The complete nucleotide sequence (155 884 bp) of tobacco (Nicotiana tabacum var. Bright Yellow 4) chloroplast DNA has been determined. It contains two copies of an identical 25 339 bp inverted repeat, which are separated by a 86 684 bp and a 18 482 bp single-copy region. The genes for 4 different rRNAs, 30 different tRNAs, 39 different proteins and 11 other predicted protein coding genes have been located. Among them, 15 genes contain introns. Blot hybridization revealed that all rRNA and tRNA genes and 27 protein genes so far analyzed are transcribed in the chloroplast and that primary transcripts of the split genes hitherto examined are spliced. Five sequences coding for proteins homologous to components of the respiratory-chain NADH dehydrogenase from human mitochondria have been found. The 30 tRNAs predicted from their genes are sufficient to read all codons if the ''two out of three'' and ''U:N wobble'' mechanisms operate in the chloroplast. Two sequences which autonomously replicate in yeast have also been mapped. The sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.