Colonization of Paracoccus sp QCT6 and Enhancement of Metribuzin Degradation in Maize Rhizosphere Soil
Colonization of Paracoccus sp QCT6 and Enhancement of Metribuzin Degradation in Maize Rhizosphere Soil
复制标题
玉米根际土壤中副球菌 QCT6 的定殖和赛克津降解的增强
DOI：
10.1007/s00284-017-1360-5
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发表时间：
2018-02-01
影响因子：
2.6
通讯作者：
Song, Man
中科院分区：
文献类型：
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作者：
Huang, Xing;Zhang, Hao;Song, Man
从嗪草酮污染土壤中筛选到一株嗪草酮降解菌QCT 6。通过生理生化试验和16 S rRNA基因系统发育分析，将该菌株鉴定为副球菌属（Paracoccus sp.）在液体培养中，菌株QCT 6对50 mg/L嗪草酮的去除率为86.4%。QCT 6降解嗪草酮的产物被鉴定为脱氨基嗪草酮，其对玉米生长的药害降低。用gfp基因标记QCT 6后，在激光共聚焦显微镜下直接观察其定殖和分布。QCT 6菌株在玉米根际土壤上表现出良好的定殖能力，并在根际土壤中保持至少20天。用GFP标记的QCT 6根际灌根后，15 mg/L嗪草酮从玉米根际土壤中去除了75.7%。该菌株在嗪草酮污染土壤的原位生物修复中具有很好的应用前景。
Strain QCT6, capable of degrading metribuzin, was isolated from metribuzin-contaminated soil. The isolate was identified as Paracoccus sp. according to its physiological characteristics, biochemical tests, and 16S rRNA gene phylogenetic analysis. In liquid culture, 86.4% of 50 mg/L metribuzin was removed by strain QCT6 after incubation for 7 days. The product of metribuzin degradation by QCT6 was identified as deamino-metribuzin, which has reduced phytotoxicity on the growth of maize. After being marked with the gfp gene, the colonization and distribution of gfp-tagged QCT6 were directly observed with a confocal laser scanning microscope. The QCT6 strain showed good colonization ability on maize roots and was maintained for at least 20 days in rhizosphere soil. After root irrigation with gfp-tagged QCT6, 75.7% of 15 mg/L metribuzin was removed from the maize rhizosphere soil. This metribuzin-degrading strain QCT6 has strong potential applications for in situ bioremediation of soil contaminated with metribuzin.