DNA methylation modulates H19 and IGF2 expression in porcine female eye.
DNA methylation modulates H19 and IGF2 expression in porcine female eye.
复制标题
DNA甲基化调节母猪眼中H19和IGF2的表达
DOI：
10.1590/1678-4685-gmb-2016-0194
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发表时间：
2017-01
影响因子：
2.1
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Wang D;Wang G;Yang H;Liu H;Li C;Li X;Lin C;Song Y;Li Z;Liu D
H19/IGF 2的性二态表达在进化上是保守的。为了研究H19/IGF 2在雌性猪眼中的表达是否具有性别依赖性，使用定量实时PCR（qPCR）和亚硫酸氢盐测序PCR（BSP）评估基因表达和甲基化状态。我们假设H19/IGF 2可能在女性眼睛中表现出不同的DNA甲基化状态。为了评估我们的假设，使用孤雌生殖（PA）细胞通过qPCR和BSP进行分析。我们的研究结果表明，H19和IGF 2在女性眼睛中的过度表达与男性眼睛相比（分别为3倍和2倍）。我们观察到H19差异甲基化区域（DMR）的正常单等位基因甲基化模式。与H19 DMR相比，IGF 2 DMR在眼睛中显示出不同的甲基化模式。总之，这些结果表明，H19/IGF 2的表达升高是由特定的染色质结构引起的，该结构由女性眼中IGF 2 DMR的DNA甲基化状态调节。
Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye.