Dual-axis confocal microscope for high-resolution in vivo imaging
Dual-axis confocal microscope for high-resolution in vivo imaging
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DOI：
10.1364/ol.28.000414
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发表时间：
2003-03-15
期刊：
影响因子：
3.6
通讯作者：
Kino, GS
中科院分区：
文献类型：
--
作者：
Wang, TD;Mandella, MJ;Kino, GS
我们描述了一种新型的共聚焦显微镜，它使用独立的低数值孔径物镜，照明轴和采集轴从中线以角θ相交。该结构在散射介质中采集图像，具有横向和轴向分辨率高、工作距离长、视场大、散射光噪声小的特点。我们在自由空间中分别测量了1.3微米和2.1微米的横向和轴向(FWHM)分辨率，并证实了切除的食道粘膜的亚细胞分辨率。光学器件可以被缩放到毫米尺寸，并且光纤耦合用于在活体内收集高分辨率图像。(C)2003年美国光学学会。
We describe a novel confocal microscope that uses separate low-numerical-aperture objectives with the illumination and collection axes crossed at angle theta from the midline. This architecture collects images in scattering media with high transverse and axial resolution, long working distance, large field of view, and reduced noise from scattered light. We measured transverse and axial (FWHM) resolution of 1.3 and 2.1 mum, respectively, in free space, and confirm subcellular resolution in excised esophageal mucosa. The optics may be scaled to millimeter dimensions and fiber coupled for collection of high-resolution images in vivo. (C) 2003 Optical Society of America.