Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year.
Colonisation with ESBL-producing and carbapenemase-producing Enterobacteriaceae, vancomycin-resistant enterococci, and meticillin-resistant Staphylococcus aureus in a long-term care facility over one year.
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DOI：
10.1186/s12879-015-0880-5
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发表时间：
2015-04-01
影响因子：
3.7
通讯作者：
Morris D
中科院分区：
文献类型：
--
作者：
Ludden C;Cormican M;Vellinga A;Johnson JR;Austin B;Morris D
本研究检查了长期护理机构（LTCF）的居民中抗菌药物耐药微生物的定植和特征，包括产超广谱β-内酰胺酶（ESBL）肠杆菌科菌株的菌株持久性和分子多样性。招募了64名LTCF居民（基线时51名，年内13名）。收集依赖程度、住院和抗菌药物处方的数据。使用显色琼脂每季度检查一次鼻腔和直肠拭子以及导尿管尿液标本中的产ESBL肠杆菌科、产碳青霉烯酶肠杆菌科（CPE）、万古霉素耐药肠球菌（VRE）和甲氧西林耐药S.金黄色葡萄球菌（MRSA）。所有产ESBL的E.通过脉冲场凝胶电泳（PFGE）和PCR对大肠杆菌（ESBL-EC）进行表征，以评估序列类型（ST）ST 131、其耐药相关H30和H30-Rx亚克隆以及blaCTX-M、blaTEM、blaSHV和blaOXA-1。按微生物划分，定植的居民总数如下：ESBL-EC，35（55%）; MRSA，17（27%）;产ESBL的K。肺炎克雷伯菌（ESBL-KP）5例（8%），万古霉素耐药菌2例（3%），细胞病变0例（0%）。所有98株ESBL-EC分离株均为H30-Rx ST 131，blaCTX-M-组1。通过PFGE，一组91例ESBL-EC（来自33名参与者）具有≥ 85%的相似性，并且与英国流行株A/国际脉冲型PFGE 812相似。个体居民ESBL-EC的发生顺序密切相关。来自5名参与者的6株ESBL-KP分离株具有blaCTX-M-1组，并且通过PFGE密切相关。ESBL和MRSA的定植与LTCF内的位置和先前暴露于抗菌剂有关。在LTCF居民中，ESBL-EC和MRSA定植很常见。所有ESBL-EC均为H30-Rx ST 131，符合克隆播散。
This study examined colonisation with and characteristics of antimicrobial-resistant organisms among residents of a long-term care facility (LTCF) over one year, including strain persistence and molecular diversity among isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae. Sixty-four residents of a LTCF were recruited (51 at baseline, 13 during the year). Data on dependency levels, hospitalisations, and antimicrobial prescribing were collected. Nasal and rectal swabs and catheter urine specimens were examined quarterly, using chromogenic agars, for ESBL-producing Enterobacteriaceae, carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE), and meticillin-resistant S. aureus (MRSA). All ESBL-producing E. coli (ESBL-EC) were characterised by pulsed-field gel electrophoresis (PFGE) and PCR to assess for sequence type (ST) ST131, its resistance-associated H30 and H30-Rx subclones, and blaCTX-M,blaTEM,blaSHV, and blaOXA-1. The overall number of residents colonised, by organism, was as follows: ESBL-EC, 35 (55%); MRSA, 17 (27%); ESBL-producing K. pneumoniae (ESBL-KP), 5 (8%); VRE, 2 (3%) and CPE, 0 (0%). All 98 ESBL-EC isolates were H30-Rx ST131, with blaCTX-M-group 1. By PFGE, a group of 91 ESBL-EC (from 33 participants) had ≥ 85% similar profiles and resembled UK epidemic strain A/ international pulsotype PFGE812. Sequential ESBL-EC from individual residents were closely related. Six ESBL-KP isolates, from five participants, had blaCTX-M-group 1 and by PFGE were closely related. Colonisation with ESBL and MRSA was associated with location within the LTCF and previous exposure to antimicrobials. Among LTCF residents, colonisation with ESBL-EC and MRSA was common. All ESBL-EC were H30-Rx ST131, consistent with clonal dissemination.
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影响因子：
4.5
作者：
Benenson, Shmuel;Cohen, Matan J.;Moses, Allon E.
通讯作者：
Moses, Allon E.
DOI：
10.4321/s1886-36552013000200006
发表时间：
2013-04
期刊：
Pharmacy practice
影响因子：
--
作者：
Bailey AM;Weant KA;Baker SN
通讯作者：
Baker SN
影响因子：
4.5
作者：
Banerjee, Ritu;Johnston, Brian;Johnson, James R.
通讯作者：
Johnson, James R.
DOI：
10.1093/cid/cis581
发表时间：
2012-10
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Hilty M;Betsch BY;Bögli-Stuber K;Heiniger N;Stadler M;Küffer M;Kronenberg A;Rohrer C;Aebi S;Endimiani A;Droz S;Mühlemann K
通讯作者：
Mühlemann K
影响因子：
14.2
作者：
Breurec, S.;Guessennd, N.;Brisse, S.
通讯作者：
Brisse, S.