TRANSFORMATION OF YEAST
TRANSFORMATION OF YEAST
复制标题
DOI：
10.1073/pnas.75.4.1929
复制
发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
HINNEN, A;HICKS, JB;FINK, GR
通过使用携带酵母leu 2基因的嵌合ColE 1质粒[来自大肠杆菌]将稳定的leu 2-酵母[酿酒酵母]菌株转化为LEU 2+。最近开发的杂交和限制性内切酶作图技术被用来直接证明转化DNA在酵母基因组中的存在，并确定所引入的序列的排列。ColE 1 DNA沿着酵母序列可以整合到酵母染色体中。这种整合可以是附加的或替代的。细菌质粒序列，一旦整合，表现为一个简单的孟德尔元件。确定每个新引入的LEU 2+等位基因与原始leu 2-等位基因的遗传连锁关系。转化序列不仅整合在leu 2区域，而且整合在几个其他染色体位置。
A stable leu2- yeast [Saccharomyces cerevisiae] strain was transformed to LEU2+ by using a chimeric ColE1 plasmid [from Escherichia coli] carrying the yeast leu2 gene. Recently developed hybridization and restriction endonuclease mapping techniques were used to demonstrate directly the presence of the transforming DNA in the yeast genome and to determine the arrangement of the sequences that were introduced. ColE1 DNA along with the yeast sequences can integrate into the yeast chromosomes. This integration may be additive or substitutive. The bacterial plasmid sequences, once integrated, behave as a simple Mendelian element. The genetic linkage relationships for each newly introduced LEU2+ allele with the original leu2- allele were determined. The transforming sequences integrate not only in the leu2 region, but also in several other chromosomal locations.