Analyses of Human Papillomavirus Genotypes and Viral Loads in Anogenital Warts
Analyses of Human Papillomavirus Genotypes and Viral Loads in Anogenital Warts
复制标题
DOI：
10.1002/jmv.22111
复制
发表时间：
2011-08-01
影响因子：
12.7
通讯作者：
Goon, Peter K. C.
中科院分区：
文献类型：
--
作者：
Ball, Siolian L. R.;Winder, David M.;Goon, Peter K. C.
尖锐湿疣（生殖器疣）是最常见的性传播病毒性疾病。这些病变是由粘膜人乳头瘤病毒（HPV）感染引起的。然而，这些病变的分子发病机制所涉及的HPV毒株分布的信息有限。为了解决这个问题，在从参加疣诊所的31名患者获得的疣组织中确定了菌株流行率和多种HPV感染的频率。将这些病变一分为二，并进行平行的DNA和mRNA提取。HPV类型的患病率和多重感染的发生率由Roche线性阵列检测确定。qPCR比较HPV 6、11、16和18型病毒载量，RT-qPCR测量HPV 6和11 E6基因组表达水平。这些样本中有71%感染了多种HPV。只有一个样本对HPV 6或11 DNA呈阴性。48%的样本为高危（致癌）HPV阳性。结果表明，组织中的多重感染是频繁的，随后对HPV 6和11 E6 DNA病毒载量的分析表明，其他HPV可能引起病变。对HPV 6/11 E6 mRNA水平的进一步分析表明，HPV 6 E6 DNA病毒载量与HPV 6或11 E6 mRNA相对水平之间没有明显关系，因此质疑病毒载量与病变因果关系的相关性。J. Med. Virol. 83：1345-1350，2011. (C)2011 Wiley-Liss，Inc.
Condylomata acuminata (genital warts) are the most common sexually transmitted viral diseases. These lesions are caused by infection with mucosal human papillomaviruses (HPVs). However, there is limited information on HPV strain distribution involved in the molecular pathogenesis of these lesions. To address this, the strain prevalence and the frequency of multiple HPV infections were determined in wart tissue obtained from 31 patients attending a wart clinic. These lesions were bisected and subjected to parallel DNA and mRNA extractions. HPV-type prevalence and incidence of multiple infections were determined by the Roche Linear Array assay. qPCR compared HPV 6, 11, 16, and 18 viral loads and RT-qPCR measured HPV 6 and 11 E6 genomic expression levels. Seventy-one percent of these samples were infected with multiple HPVs. Only one sample was negative for HPV 6 or 11 DNA. Forty-eight percent of samples were positive for a high risk (oncogenic) HPV. The results show that multiple infections in tissue are frequent and the subsequent analysis of HPV 6 and 11 E6 DNA viral loads suggested that other HPVs could be causing lesions. Further analysis of HPV 6/11 E6 mRNA levels showed that there was no discernable relationship between HPV 6 E6 DNA viral load and relative HPV 6 or 11 E6 mRNA levels thereby questioning the relevance of viral load to lesion causality. J. Med. Virol. 83:1345-1350, 2011. (C) 2011 Wiley-Liss, Inc.