Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment
Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment
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DOI：
10.1016/j.lwt.2018.09.059
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发表时间：
2019
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通讯作者：
M. Watanabe;Chikako Yamada;I. Maeda;Charin Techapun;Ampin Kuntiya;N. Leksawasdi;Phisit Seesuriyachan;Thanongsak Chaiyaso;S. Takenaka;Tadahiko Shiono;K. Nakamura;S. Endo
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作者：
M. Watanabe;Chikako Yamada;I. Maeda;Charin Techapun;Ampin Kuntiya;N. Leksawasdi;Phisit Seesuriyachan;Thanongsak Chaiyaso;S. Takenaka;Tadahiko Shiono;K. Nakamura;S. Endo
采用等电子沉淀法和电解水处理(IP-EWT)相结合的工艺同时回收热稳定脱脂米糠中的蛋白质和磷化合物。采用IP-EWT两步法提取浓缩蛋白，蛋白质含量最高(65.1w/w%)，蛋白质回收率在50%以上。氨基酸组成分析表明，蛋白质组分的氨基酸价值(76.6%)高于商品谷物，如大米(61%)、小麦(39%)和玉米(31%)。此外，根据SDS-PAGE和免疫印迹分析，无论是HSDFRB还是IP-EWT提取的蛋白质组分中都没有检测到大米过敏蛋白和重金属。这些结果表明，作为一种生态友好的工艺，这种组合(IP-EWT)工艺适合于从HSDFRB中实用地回收高浓度和安全的蛋白质，而不需要使用酶或化学物质，如有机溶剂、缓冲剂和表面活性剂。
A combination of isoelectronic precipitation and electrolyzed water treatment (IP-EWT) process was use for simultaneous recovery of protein and phosphorus compounds from heat-stabilized defatted rice bran (HSDFRB). The highest protein content (65.1 w/w%) recovery ratio (over 50%) of protein concentrate were attained by two-stage protein extraction with IP-EWT. Analysis of amino-acid composition demonstrated excellent amino acid value (76.6%) in the protein fraction than those of commercial cereals such as rice (61%), wheat (39%), and corn (31%). In addition, according to SDS-PAGE and immunoblotting analysis, neither rice allergenic protein nor heavy metals were detected in either HSDFRB or protein fractions extracted by IP-EWT. These results suggest that as an eco-friendly process, this combined (IP-EWT) process is suitable for practical recovery of highly concentrated and safe protein from HSDFRB without using enzymes or chemicals such as organic solvents, buffering agents, and surfactants.