Insight into the Mechanism of Reproductive Dysfunction Caused by Neonicotinoid Pesticides
Insight into the Mechanism of Reproductive Dysfunction Caused by Neonicotinoid Pesticides
复制标题
DOI：
10.1248/bpb.b14-00359
复制
发表时间：
2014-09-01
影响因子：
2
通讯作者：
Kitagawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Hoshi, Nobuhiko;Hirano, Tetsushi;Kitagawa, Hiroshi
新烟碱类杀虫剂是20世纪90年代开发的一种具有选择性毒性的杀虫剂，后来被发现会导致实验动物的生殖异常。日本正在尝试保护濒临灭绝的动物，包括日本朱鹮，并且存在新烟碱类杀虫剂是否会影响这种鸟的繁殖的问题，因为它们是在其栖息地使用的。因此，我们作为实验动物，研究了每日口服新烟碱类噻虫胺（CTD）是否对成熟雄性鹌鹑或年轻雄性和雌性鹌鹑的生殖功能有任何有害影响。生精小管中的空泡化和具有片段化DNA的生殖细胞的数量，以及肝细胞中空泡的数量和大小，均呈剂量依赖性增加。卵巢产生黄体酮的颗粒细胞组织学异常。各组之间的产蛋率和胚胎重量存在显着差异。保护机体免受氧化损伤的谷胱甘肽过氧化物酶 4 (GPx4) 和锰超氧化物歧化酶 (Mn-SOD) 显示出剂量依赖性下降。因此，新烟碱类杀虫剂可能通过氧化应激影响鸟类的生殖系统，反映了活性氧（ROS）的产生与生物系统轻松解毒反应中间体或轻松修复所造成损伤的能力之间的不平衡。根据我们的研究，佐渡岛在不使用新烟碱类杀虫剂的情况下成功地在野外繁殖了朱鹮。
Neonicotinoids, which were developed in the 1990s as an insecticide having selective toxicity, were later found to cause reproductive abnormalities in experimental animals. In Japan there is an attempt to preserve endangered animals, including the Japanese crested ibis, and there is a question of whether neonicotinoids affect the reproduction of this bird, since they are used in its habitat. Hence, we investigated whether the daily oral administration of the neonicotinoid clothianidin (CTD) has any deleterious effects on the reproductive function of mature male only or both young male and female quails as experimental animals. Vacuolization and the number of germ cells having fragmented DNA in seminiferous tubules, as well as the number and size of vacuoles in hepatocytes, increased dose-dependently. The ovaries showed abnormal histology in the granulosa cells, which produce progesterone. There were significant differences in egg-laying rates and embryo weights between the groups. Glutathione Peroxidase 4 (GPx4) and Manganese Superoxide Dismutase (Mn-SOD), which protect the organism from oxidative damage, showed a dose-dependent decrease. Thus, it is possible neonicotinoids affect the bird's reproductive system through oxidative stress, reflecting an imbalance between the production of reactive oxygen species (ROS) and a biological system's ability to readily detoxify the reactive intermediates or easily repair the resulting damage. Responding to our study, Sado Island has since succeeded in breeding Japanese crested ibis in the wild without the use of neonicotinoids.