Characterization of CNS precursor subtypes and radial glia
Characterization of CNS precursor subtypes and radial glia
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DOI：
10.1006/dbio.2000.9962
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Götz, M
中科院分区：
文献类型：
--
作者：
Hartfuss, E;Galli, R;Götz, M
放射状胶质细胞作为迁移神经元的导向细胞的作用已得到充分确立，然而它们作为前体细胞的作用却鲜为人知。在此我们研究了发育过程中小鼠端脑内放射状胶质细胞的组成及其增殖情况。我们发现，在整个神经发生过程中，几乎所有的放射状胶质细胞都在增殖。通过对RC2、星形胶质细胞特异性谷氨酸转运体（GLAST）和脑脂质结合蛋白（BLBP）抗原进行免疫染色，可将它们分为三个不同的亚群。此外，RC2、GLAST和BLBP抗血清可标记具有不同形态的前体细胞，从而几乎涵盖了发育中的大脑皮质的整个祖细胞群。通过这些抗原的差异表达所确定的亚群在转录因子表达和细胞周期特征方面也存在差异。而且，BLBP的含量似乎与后代的命运相关。BLBP阴性前体细胞仅在神经发生期间被检测到，并且仅在吻侧迁移流（一个持续神经发生的区域）中持续到出生后阶段。相比之下，从成年或胚胎端脑衍生的多潜能细胞（神经球培养物）富集群体对RC2、GLAST和BLBP呈免疫反应。综上所述，我们已经鉴定出中枢神经系统前体细胞的新型、功能不同的亚群。（C）2001年学术出版社
The role of radial glial cells as guides for migrating neurons is well established, whereas their role as precursor cells is less understood. Here we examined the composition of radial glial cells and their proliferation in the mouse telencephalon during development. We found that almost all radial glial cells proliferate throughout neurogenesis. They consist of three distinct subsets identified by immunostaining for the antigens RC2, the astrocyte-specific glutamate transporter (GLAST), and the brain-lipid-binding protein (BLBP). In addition, RC2, GLAST, and BLBP antisera label precursor cells with different morphologies and thereby cover almost the entire progenitor pool in the developing cerebral cortex. The subsets identified by differential expression of these antigens differ also in their transcription factor expression and cell cycle characteristics. Moreover, the content of BLBP seems correlated to the fate of the progeny. BLBP-negative precursors are detected only during neurogenesis and persist into postnatal stages solely in the rostral migratory stream, a region of ongoing neurogenesis. In contrast, an enriched population of multipotential cells, neurosphere cultures derived from the adult or embryonic telencephalon, is immunoreactive for RC2, GLAST, and BLBP. Taken together, we have identified novel, functionally distinct subsets of CNS precursor cells. (C) 2001 Academic Press.