LIFE-SPAN OF HUMAN-LYMPHOCYTES ESTIMATED DURING A 6 YEAR CYTOGENETIC FOLLOW-UP OF INDIVIDUALS ACCIDENTALLY EXPOSED IN THE 1987 RADIOLOGICAL ACCIDENT IN BRAZIL
LIFE-SPAN OF HUMAN-LYMPHOCYTES ESTIMATED DURING A 6 YEAR CYTOGENETIC FOLLOW-UP OF INDIVIDUALS ACCIDENTALLY EXPOSED IN THE 1987 RADIOLOGICAL ACCIDENT IN BRAZIL
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DOI：
10.1016/0027-5107(95)00049-o
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发表时间：
1995-09-01
影响因子：
2.3
通讯作者：
NATARAJAN, AT
中科院分区：
文献类型：
--
作者：
RAMALHO, AT;CURADO, MP;NATARAJAN, AT
在1987年9月戈亚尼亚市（巴西）发生放射性事故后，开始对15名受照患者进行细胞遗传学随访，目的是观察含有双着丝粒和环状畸变的淋巴细胞的平均寿命。结果表明，不稳定像差的消失率服从二项指数函数。暴露后470天，畸变频率迅速下降。470天后，消失速度非常缓慢。这些结果可能反映了人类淋巴细胞的不同亚群，具有不同的寿命。在高度接触组（剂量超过1戈伊）中，估计在接触后的最初阶段（最多470天），双着丝粒体和环的平均半衰期为110天。该值明显短于文献中报道的通常接受的3年值。对个体半衰期与生物学参数（如性别、年龄、关键期内显示的白细胞减少水平、吸收剂量（染色体畸变的初始频率）和骨髓刺激因子（rHuGM-CSF）给药）之间可能的相关性进行了统计分析。这些参数均未显示与染色体畸变消失的半衰期相关。对于接受不到1戈伊的个体，畸变的消失较慢，在照射后470天内的半衰期为160天。不稳定染色体畸变的平均消失函数推断，适用于事故情况下，其中有一个血液采样延迟。
Following the radiological accident which occurred in the city of Goiania (Brazil), in September 1987, a cytogenetic follow-up of 15 exposed patients was started, aiming to observe the mean lifetime of lymphocytes containing dicentric and ring aberrations. The results suggest that the disappearance rate of unstable aberrations follows a two-term exponential function. Up to 470 days after exposure, there is a rapid fall in the aberration frequency. After 470 days, the disappearance rate is very slow. These results may reflect different subpopulations of human lymphocytes, with different lifespans. The estimated average half-time of elimination of dicentrics and rings among the highly exposed group (doses above 1 Gy) was 110 days for the initial period after the exposure (up to 470 days). This value is significantly shorter than the usually accepted value of 3 years reported in the literature. Statistical analysis of possible correlations between the individual half-times and biological parameters, such as sex, age, leukopenia level shown during the critical period, absorbed dose (initial frequency of chromosomal aberrations) and the administration of the bone marrow stimulating factor (rHuGM-CSF) was performed. None of these parameters showed a correlation with the half-time of disappearance of chromosomal aberrations. For the individuals who had received less than 1 Gy the disappearance of aberrations was slower, with a half-time of 160 days during the period up to 470 days after exposure. Mean disappearance functions of unstable chromosome aberrations were inferred, to be applied in accident situations in which there is a blood sampling delay.