PCR designer for restriction analysis of various types of sequence mutation
PCR designer for restriction analysis of various types of sequence mutation
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DOI：
10.1093/bioinformatics/18.12.1688
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发表时间：
2002-12-01
期刊：
影响因子：
5.8
通讯作者：
Ye, S
中科院分区：
文献类型：
--
作者：
Ke, XY;Collins, A;Ye, S
限制性内切分析广泛用于检测基因突变，如插入、缺失和单核苷酸多态性（snp）。虽然这些突变位点有时会出现一些天然的限制性内切位点来区分野生型和突变型序列，但为了产生人工限制性内切片段长度多态性（RFLPs），通常需要错配。在本报告中，描述了一个计算机程序筛选合适的限制性内切酶，在适当和必要时引入错配，利用所选限制性内切酶的信息、它们的识别序列和位置以及有关错配的信息设计引物。该程序由WWW网络界面支持，旨在在线使用。
Restriction analysis is widely used to detect gene mutations such as insertions, deletions and single nucleotide polymorphisms (SNPs). Although such mutation sites sometimes present some natural restriction sites to differentiate the wild-type and mutant sequences, mismatches are often needed in order to create artificial restriction fragment length polymorphisms (RFLPs). In this report, a computer program is described that screens for suitable restriction enzymes, introducing mismatches where appropriate and when necessary, designs primers using the information of the selected restriction enzymes, their recognition sequence and locations as well as the information about the mismatches if any. The program, supported by a WWW web interface, is intended to be used online.