Microprobe analysis of maturation-related elemental changes in rat parotid secretory granules.
Microprobe analysis of maturation-related elemental changes in rat parotid secretory granules.
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大鼠腮腺分泌颗粒成熟相关元素变化的微探针分析。
DOI：
10.1152/ajpcell.1991.261.6.c1033
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Johnson,DE
中科院分区：
文献类型：
--
作者：
Wong,JG;Izutsu,KT;Robinovitch,MR;Iversen,JM;Cantino,ME;Johnson,DE
电子探针x射线微量分析用于定量分泌颗粒成熟过程中发生的元素和质量变化。单次注射异丙肾上腺素可刺激大鼠腮腺腺泡细胞分泌颗粒的耗竭。不同成熟阶段的颗粒随着时间的推移在细胞内重新积累。干质量测量显示，分泌物质在成熟颗粒内浓缩约两倍。几乎所有质量浓度的增加都可以归因于水空间的减少。数据显示，钠、钾、氯和水在成熟过程中都从分泌颗粒中流出。颗粒S含量与成熟度呈正相关。因此，在成熟过程中，颗粒元素和水分含量发生了显著变化。
Electron probe X-ray microanalysis was use to quantitate the elemental and mass changes that take place during the secretory granule maturation process. A single injection of isoproterenol stimulated the depletion of secretory granules from rat parotid acinar cells. Granules at different stages of maturation were analyzed as they reaccumulated within the cells over time. Dry mass measurements revealed that secretory material becomes concentrated about twofold within maturing granules. Nearly all of the increase in mass concentration could be attributed to a reduction in water space. Data are presented that indicate that Na, K, Cl, and water all efflux from secretory granules during maturation. In contrast, granule S content is positively correlated with maturation. Hence, significant changes in granule elemental and water contents occur during the maturation process.