Effects of ageing and oestrogen on the localization of immunoglobulin-containing cells in the chicken ovary
Effects of ageing and oestrogen on the localization of immunoglobulin-containing cells in the chicken ovary
复制标题
DOI：
10.1530/jrf.0.1140011
复制
发表时间：
1998-09-01
期刊：
影响因子：
--
通讯作者：
Tamura, T
中科院分区：
文献类型：
--
作者：
Barua, A;Yoshimura, Y;Tamura, T
鸡卵巢中的免疫球蛋白对于通过卵将免疫传递给雏鸡和保护卵巢免受感染是重要的。本研究的目的是研究衰老和雌激素对鸡卵巢中含Ig细胞群体的影响。对未成熟、年轻和年老产蛋鸡的卵巢组织以及用己烯雌酚（DES）、孕酮或芝麻油（溶媒）处理的未成熟鸡的卵巢组织进行石蜡切片。通过间接免疫染色法对切片进行IgG、IgM和伊加染色，并在光学显微镜下分析每种IG阳性的细胞群。幼龄蛋鸡卵巢间质IgG、IgM、伊加阳性细胞数显著高于幼龄和老龄蛋鸡（P
Immunoglobulins in the chicken ovary are important for transfer of immunity to chicks through the egg and for protection of the ovary from infection. The aim of this study was to examine the effects of ageing and oestrogen on the population of Ig-containing cells in the chicken ovary. The ovarian tissue of immature, young laying and old laying hens and that of immature birds treated with diethylstilboestrol (DES), progesterone or sesame oil (vehicle) was processed for paraffin wax sections. The sections were stained for IgG, IgM and IgA by an indirect immunostaining method and the population of cells positive for each Ig was analysed under a light microscope. The number of cells positive for IgG, IgM and IgA was significantly greater in the ovarian stromal tissue of young laying hens than in immature or old laying hens (P