THE EFFECT OF INHIBITING SCHWANN-CELL MITOSIS ON THE RE-INNERVATION OF ACELLULAR AUTOGRAFTS IN THE PERIPHERAL NERVOUS-SYSTEM OF THE MOUSE
THE EFFECT OF INHIBITING SCHWANN-CELL MITOSIS ON THE RE-INNERVATION OF ACELLULAR AUTOGRAFTS IN THE PERIPHERAL NERVOUS-SYSTEM OF THE MOUSE
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DOI：
10.1111/j.1365-2990.1986.tb00151.x
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发表时间：
1986-07-01
影响因子：
5
通讯作者：
HALL, SM
中科院分区：
文献类型：
--
作者：
HALL, SM
神经内注射丝裂霉素C可抑制坐骨神经横断近端的反应性胶质细胞增生，丝裂霉素C是一种已知的抗有丝分裂药物，可在横断、挤压或脱髓鞘后阻止雪旺细胞的分裂。丝裂霉素C处理的近端残端随后被缝合到细胞或无细胞自体移植物(0.5厘米长)上，并在术后5周内检查移植物内和移植物内的轴突生长情况。神经突长入细胞自体移植物，并与移植物内雪旺细胞的居留群体相关，到缝合后5周，大多数雪旺细胞基板管已形成良好的再髓鞘形成。与此形成鲜明对比的是，在这段时间里，很少有神经突起生长到无细胞移植物中，而且在轴突和雪旺细胞出现的地方，它们往往被聚集在“迷你肌束”中。结果表明，近端残端突起的生长依赖于雪旺细胞的活跃参与。
Reactive gliosis in the zone immediately proximal to transection of the sciatic nerve has been inhibited by intraneural injection of mitomycin C, an anti-mitotic agent known to arrest Schwann cell division after transection, crush or demyelination. Mitomycin C-pretreated proximal stumps were subsequently sutured to cellular or acellular autografts (o.5 cm long) and neurite growth into and within the grafts was examined during a 5-week post-operative period. Neurites grew into cellular autografts and became associated with the resident population of Schwann cells within the grafts, to the extent that remyelination was well established in the majority of Schwann cell basal lamina tubes by week 5 post-suture. In marked contrast, very few neurites grew into acellular grafts during this time, and where axons and Schwann cells were seen they tended to be grouped in ''minifascicles''. The results suggest that neurite outgrowth from proximal stumps is dependent upon active Schwann cell participation.