The heterogeneous growth cone glycoprotein gp93 is identical to the signal regulatory protein SIRPalpha/SHPS-1/BIT.
The heterogeneous growth cone glycoprotein gp93 is identical to the signal regulatory protein SIRPalpha/SHPS-1/BIT.
复制标题
异质生长锥糖蛋白 gp93 与信号调节蛋白 SIRPalpha/SHPS-1/BIT 相同。
DOI：
10.1046/j.1471-4159.2003.01810.x
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发表时间：
2003
影响因子：
4.7
通讯作者：
Pfenninger,KarlH
中科院分区：
文献类型：
--
作者：
Wang,XiaoxinX;Dangott,LawrenceJ;Pfenninger,KarlH
生长锥gp 93是一种高度异质性的膜糖蛋白，分子量约为93 kDa。 从成年大鼠脑中纯化并进行微测序。gp 93的四个不同肽段的序列与“信号调节蛋白”SIRPα（也称为SHPS-1、BIT或P84）的序列相匹配，SIRP α是IG超家族成员。SIRPα含有一个胞质尾区，该尾区是酪氨酸激酶底物并结合蛋白酪氨酸磷酸酶SHP-2。SIRPα和gp 93也具有免疫化学交叉反应性。使用PCR策略来确定脑中gp 93/SIRPα异质性是否取决于不同转录物的存在，从而确定序列异质性。然而，我们只观察到一个完整的转录本。还检测到短剪接变体。这些数据将gp 93鉴定为IG超家族成员SIRPα。与我们之前的结果一起，数据还表明，在大鼠脑中，gp 93/SIRPα异质性是差异糖基化（加上磷酸化）的结果，而不是序列异质性。
Growth cone gp93 is a highly heterogeneous membrane glycoprotein with anMrof about 93 kDa. It was purified from adult rat brain and microsequenced. The sequences of four different peptide fragments of gp93 matched those of the ‘signal regulatory protein’ SIRPα (also known as SHPS‐1, BIT or P84), an Ig superfamily member. SIRPα contains a cytoplasmic tail that is a tyrosine kinase substrate and binds the protein tyrosine phosphatase SHP‐2. SIRPα and gp93 also were immunochemically cross‐reactive. A PCR strategy was used to determine whether gp93/SIRPα heterogeneity in the brain depended upon the presence of different transcripts and, thus, sequence heterogeneity. However, we observed only a single full‐length transcript. A short splice variant also was detected. These data identify gp93 as the Ig superfamily member SIRPα. Together with our previous results, the data also demonstrate that, in rat brain, gp93/SIRPα heterogeneity is the result of differential glycosylation (plus phosphorylation), rather than sequence heterogeneity.