Genetic structure of pine sawyer Monochamus alternatus (Coleoptera: Cerambycidae) populations in northeast Asia:: Consequences of the spread of pine wilt disease
Genetic structure of pine sawyer Monochamus alternatus (Coleoptera: Cerambycidae) populations in northeast Asia:: Consequences of the spread of pine wilt disease
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DOI：
10.1603/0046-225x-35.2.569
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发表时间：
2006-04-01
影响因子：
1.7
通讯作者：
Hogetsu, T
中科院分区：
文献类型：
--
作者：
Kawai, M;Shoda-Kagaya, E;Hogetsu, T
为了解松材线虫的定殖动态、进一步入侵的可能性和物种分化的可能性，我们对松材线虫Monochamus ternatus(Hope)的系统地理和遗传多样性进行了研究。本种是松材线虫松材线虫(Steiner And Buhrer)的主要媒介，该线虫是日本松材线虫病的病原体。利用线粒体DNA序列和微卫星序列对其遗传结构进行了研究。系统发育分析揭示了松墨天牛的两个不同的谱系。这两个分支的单倍型分布之间没有明确的界限。合并应该通过种群细分来扩大。这两个不同的种群也可能融合在一起，而且两者都是完全饱和的。对日本人群的微卫星基因分型分析显示出复杂的遗传结构。总体种群分化(F-ST)的估计值与零有显著差异。这些种群被认为处于人口结构不平衡状态，或者显示出县与县之间有限的基因流动。虽然相邻种群通常具有相似的遗传组成，但在总种群中没有检测到显著的距离隔离。有人认为，种群的扩张可能不仅是由于小规模的自然扩散，还可能是由于(由人类)受侵扰的木材的重新安置而促进的长距离扩散。我们的数据表明，松墨天牛是一种能够在大范围内扩散的物种，因此，松墨天牛有可能从遥远的地区多次入侵。
We studied the phylogeography and genetic diversity of the pine sawyer Monochamus alternatus (Hope) (Coleoptera: Cerambycidae) to understand its colonization dynamics, potential for further invasion, and potential species divergence. This species is the main vector of the pine wood nematode Bursaphelenchus xylophilus (Steiner and Buhrer), which is the causative agent of pine wilt disease in Japan. The genetic structure was studied using sequences of mitochondrial DNA and microsatellites. The phylogenetic analysis revealed two distinct lineages within M. alternatus. There is no clear boundary between haplotypic distributions of the two clades. Coalescence should have been extended by population subdivision. There might also be a fusion of the two distinct populations, and both are completely saturated. Analysis of the microsatellite genotypes of populations in Japan showed a complex genetic structure. Estimates of overall population differentiation (F-ST) were significantly different from zero. The populations are thought to be at demographical nonequilibrium or to show restricted gene flow among prefectures. Although neighboring populations often had similar genetic compositions, significant isolation by distance in the total population was not detected. It is suggested that population expansion may have occurred not only by natural dispersal on a small scale, but also by long-distance dispersal likely enhanced by the relocation, (by humans) of infested wood. Our data Suggest that M. alternatus is a species capable of dispersing over a large area, and therefore, multiple invasions of M. alternatus from distant areas is a possibility.