Interferon epsilon in the reproductive tract of healthy and genital herpes simplex virus-infected pregnant women: Results of a pilot study
Interferon epsilon in the reproductive tract of healthy and genital herpes simplex virus-infected pregnant women: Results of a pilot study
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DOI：
10.1111/aji.12995
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发表时间：
2018-09-01
影响因子：
3.6
通讯作者：
Taylor, Brandie D.
中科院分区：
文献类型：
--
作者：
Nickodem, Colette;Criscitiello, Michael F.;Taylor, Brandie D.
问题最近鉴定的干扰素α（IFNe）在动物模型中可预防性传播感染，包括生殖器单纯疱疹病毒（HSV）。没有关于孕妇生殖道分泌物中IFNe的报告，关于非孕妇中IFNe的数据有限。这项试验性研究是第一个测量怀孕期间阴道和宫颈分泌物中IFNe的浓度，并比较健康和生殖器HSV感染women.Method的值研究从全球联盟预防早产和死产（GAPPS）库获得30名孕妇的阴道或宫颈标本。在妊娠早期采集宫颈样本，在整个妊娠期间采集阴道样本。酶联免疫吸附试验测定IFNe浓度（pg/mL）。IFNe的数据进行对数转换，并使用t检验和线性回归通过母体人口统计学、临床变量和HSV状态进行比较。重复测量分析探讨了整个pregnancy.ResultsAmong整个队列的趋势，在阴道或宫颈分泌物中的IFNe的头三个月浓度下降，体重指数增加（=-0. 14，P=. 0466）。HSV感染和健康妇女的阴道IFNe浓度在整个妊娠期间增加（P= 0.009）。平均阴道IFNe在整个怀孕的妇女与HSV相比，健康women（P=.0009）。这项试点调查无法得出任何明确的结论。然而，动物模型表明，IFNe可以防止性传播感染。因此，需要更大规模的研究来验证IFNe在有和没有生殖器感染的孕妇生殖道中的表达。
ProblemRecently characterized interferon epsilon (IFNe) protects against sexually transmitted infections, including genital herpes simplex virus (HSV), in animal models. There are no reports of IFNe in genital tract secretions of pregnant women, and data on IFNe in non-pregnant women are limited. This pilot study is the first to measure concentrations of IFNe in vaginal and cervical secretions during pregnancy and compare values between healthy and genital HSV-infected women.Method of StudyVaginal or cervical specimens from 30 pregnant women were obtained from the Global Alliance to Prevent Prematurity and Stillbirth (GAPPS) repository. Cervical samples were collected during the first trimester and vaginal samples across pregnancy. Enzyme-linked immunosorbent assay determined concentrations of IFNe (pg/mL). Data for IFNe were log-transformed and compared by maternal demographics, clinical variables, and HSV status using t tests and linear regression. Repeated measures analysis explored trends across pregnancy.ResultsAmong the entire cohort, first trimester concentrations of IFNe in vaginal or cervical secretions decreased as body mass index increased (=-0.14, P=.0466). Concentrations of vaginal IFNe increased across pregnancy in HSV-infected and healthy women (P=.009). Average vaginal IFNe across pregnancy was lower in women with HSV compared to healthy women (P=.0009).ConclusionInterferon epsilon increased across pregnancy, but was less abundant in women with HSV. This pilot investigation cannot make any definitive conclusions. However, animal models suggest that IFNe may protect against STIs. Thus, larger studies are required to validate expression of IFNe in the reproductive tract of pregnant women with and without genital infections.