A TESTIS-EXPRESSED ZN FINGER GENE (ZNF76) IN HUMAN 6P21.3 CENTROMERIC TO THE MHC IS CLOSELY LINKED TO THE HUMAN HOMOLOG OF THE T-COMPLEX GENE TCP-11
A TESTIS-EXPRESSED ZN FINGER GENE (ZNF76) IN HUMAN 6P21.3 CENTROMERIC TO THE MHC IS CLOSELY LINKED TO THE HUMAN HOMOLOG OF THE T-COMPLEX GENE TCP-11
复制标题
DOI：
10.1016/s0888-7543(05)80167-3
复制
发表时间：
1992-11-01
期刊：
影响因子：
4.4
通讯作者：
TROWSDALE, J
中科院分区：
文献类型：
--
作者：
RAGOUSSIS, J;SENGER, G;TROWSDALE, J
用6p 21的cDNA文库筛选获得一个睾丸表达的锌指基因ZNF 76。ZNF 76是DNA结合蛋白GLI-Krüppel家族的成员。它在小鼠中是保守的，其中睾丸中的转录在出生后第20天开始。小鼠tcp-11基因位于t复合体的远端倒位，并以与ZNF 76相同的方式进行发育调控。tcp-11的人类同源物被分离出来以进行精确的染色体定位。通过使用体细胞杂交，辐射杂交，中期和间期荧光原位杂交，和脉冲场凝胶电泳的组合，我们映射到6p21.2到6p21.3区域的两个基因，并将它们彼此之间的300 kb的DNA，约2 Mb的着丝粒的主要组织相容性复合体。
A novel testis-expressed Zn finger gene (ZNF76) was identified by screening cDNA libraries with cosmids derived from 6p21. ZNF76 is a member of the GLI-Krüppel family of DNA binding proteins. It is conserved in mouse where transcription in testis is initiated at Day 20 after birth. The mouse tcp-11 gene is located in the distal inversion of the t-complex and is developmentally regulated in the same manner as ZNF76. The human homolog of tcp-11 was isolated to allow a precise chromosomal localization. By using a combination of somatic cell hybrids, radiation hybrids, metaphase and interphase fluorescentin situhybridization, and pulsed-field gel electrophoresis, we mapped the two genes to the 6p21.2 to 6p21.3 region and linked them to each other within 300 kb of DNA, approximately 2 Mb centromeric to the major histocompatibility complex.