The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
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DOI：
10.1083/jcb.114.2.343
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发表时间：
1991-07
期刊：
影响因子：
--
通讯作者：
Butcher EC
中科院分区：
文献类型：
--
作者：
Berg EL;Robinson MK;Warnock RA;Butcher EC
淋巴细胞从血液进入淋巴器官的运输是由组织选择性淋巴细胞与毛细血管后小静脉内衬的特化内皮细胞相互作用控制的，特别是淋巴组织和慢性炎症部位中发现的高内皮小静脉（HEV）。淋巴细胞与戊型肝炎病毒的相互作用部分是由淋巴细胞归巢受体和组织特异性戊型肝炎病毒决定簇（血管地址素）介导的。用单克隆抗体MECA-79在小鼠和人的免疫组织化学中检测到一种外周淋巴结地址素（PNAd），它抑制淋巴细胞归巢淋巴结和淋巴细胞与扁桃体HEV的结合。人MECA-79抗原PNAd在分子上不同于65-kD粘膜血管地址素。SDS-PAGE显示，最丰富的碘化物质为105 kD.当亲和分离并固定在载玻片上时，MECA-79免疫分离材料以钙依赖性方式与人和小鼠淋巴细胞强烈结合。通过mAb MECA- 79、抗小鼠或人LECAM-1（外周淋巴结归巢受体，MEL-14抗原，LAM-1）的抗体以及用神经氨酸酶处理PNAd阻断结合。LECAM-1 cDNA的表达赋予转染的B细胞系PNAd结合能力。我们的结论是，LECAM-1介导淋巴细胞结合PNAd，一种相互作用，涉及凝集素活性的LECAM-1和碳水化合物决定簇的地址。
The trafficking of lymphocytes from the blood and into lymphoid organs is controlled by tissue-selective lymphocyte interactions with specialized endothelial cells lining post capillary venules, in particular the high endothelial venules (HEV) found in lymphoid tissues and sites of chronic inflammation. Lymphocyte interactions with HEV are mediated in part by lymphocyte homing receptors and tissue-specific HEV determinants, the vascular addressins. A peripheral lymph node addressin (PNAd) has been detected immunohistologically in mouse and man by monoclonal antibody MECA-79, which inhibits lymphocyte homing to lymph nodes and lymphocyte binding to lymph node and tonsillar HEV. The human MECA-79 antigen, PNAd, is molecularly distinct from the 65-kD mucosal vascular addressin. The most abundant iodinated species by SDS- PAGE is 105 kD. When affinity isolated and immobilized on glass slides, MECA-79 immunoisolated material binds human and mouse lymphocytes avidly in a calcium dependent manner. Binding is blocked by mAb MECA- 79, by antibodies against mouse or human LECAM-1 (the peripheral lymph node homing receptor, the MEL-14 antigen, LAM-1), and by treatment of PNAd with neuraminidase. Expression of LECAM-1 cDNA confers PNAd binding ability on a transfected B cell line. We conclude that LECAM-1 mediates lymphocyte binding to PNAd, an interaction that involves the lectin activity of LECAM-1 and carbohydrate determinants on the addressin.