Mapping of viral epitopes with prokaryotic expression products.
Mapping of viral epitopes with prokaryotic expression products.
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DOI：
10.1007/bf01310699
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发表时间：
1990
影响因子：
2.7
通讯作者：
van der Zeijst BA
中科院分区：
文献类型：
--
作者：
Lenstra JA;Kusters JG;van der Zeijst BA
有几种系统可用于在大肠杆菌中表达外源基因序列。我们描述了使用病毒基因片段的原核表达产物来识别指定抗体结合位点的区域。如果抗原性不依赖于天然蛋白质，而只依赖于氨基酸序列，即如果表位是连续的，这种方法尤其成功。将原核表达与合成肽的使用相结合，通常可以快速而准确地绘制表位。在病毒表面出现免疫优势序列表位似乎是一种普遍现象。
Several systems are available for the expression of foreign gene sequences inEscherichia coli. We describe the use of prokaryotic expression products of viral gene fragments in order to identify the regions that specify the binding sites of antibodies. This approach is particulary successful if the antigenicity does not depend on the native protein, but only on the amino acid sequence, i.e., if the epitope is sequential. Combining prokaryotic expression with the use of synthetic peptides often permits a fast and accurate mapping of an epitope. The occurrence of immunodominant sequential epitopes on the surface of viruses seems to be a widespread phenomenon.