Isolation and characterization of a novel human NM23-H1B gene, a different transcript of NM23-H1
Isolation and characterization of a novel human NM23-H1B gene, a different transcript of NM23-H1
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DOI：
10.1007/s100380300014
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发表时间：
2003-01-01
影响因子：
3.5
通讯作者：
Mao, YM
中科院分区：
文献类型：
--
作者：
Ni, XH;Gu, SH;Mao, YM
NM 23基因是一个显著的转移抑制基因。已经发现了八个NM 23/核苷二磷酸激酶家族的人类基因。从我们的大cDNA克隆和测序项目中，我们从18周龄的人胎脑中克隆了人NM 23-H1的不同转录本（NM 23-H1B）。该基因全长987 bp，编码177个氨基酸残基的蛋白质。与NM 23 H1相比，该cDNA在NH 2端增加了一个25个氨基酸残基。利用生物信息学分析将其定位于染色体17q21.3，结果表明该基因的第二外显子不存在于NM 23-H1中。NM 23-H1B的表达模式显示，它在正常组织（除结肠外的15种组织）中以不同水平普遍表达。我们的数据还表明，转录本在肿瘤中的表达与肿瘤分化有关：在低分化乳腺癌GI-101，胰腺癌GI-103和未分化卵巢癌GI-102中，没有表达。在低分化肺癌LX-1、GI-117中表达水平很低。高分化结肠腺癌CX-1的转录条带显著高于低分化结肠腺癌GI-112。在IV级前列腺腺癌PC 3中也发现了高转录水平。
The NM23 gene is a conspicuous metastasis-suppressor gene. Eight human genes of the NM23/ nucleoside diphosphate kinase family have been discovered. From our large cDNA cloning and sequencing project, we cloned a different transcript (NM23-H1B) of human NM23-H1 from 18-week-old human fetal brain. The 987-bp cDNA encodes a protein of 177 amino acid residues. Compared with NM23H1, the cDNA contained an additional NH2-terminal region (25 amino acid residues). It was mapped to chromosome 17q21.3 using bioinformatics analysis, which shows that the second exon does not exist in NM23-H1. The expression pattern of NM23-H1B showed that it was ubiquitously expressed in normal tissues (15 tissues except colon) at different levels. Our data also indicated that the expression of the transcript in tumors related to tumor differentiation: in poorly differentiated breast carcinoma GI-101, pancreatic adenocarcinoma GI-103, and undifferentiated ovarian carcinoma GI-102, there was no expression. In poorly differentiated lung carcinoma LX-1, lung carcinoma GI-117, the expression level was very low. The transcript band in well-differentiated colon adenocarcinoma CX-1 was significantly higher than that in poorly differentiated colon adenocarcinoma GI-112. A high transcription level was also found in grade IV prostatic adenocarcinoma PC3.