Infection of a Chlorella-like alga with the virus PBCV-1: transcriptional studies.
Infection of a Chlorella-like alga with the virus PBCV-1: transcriptional studies.
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病毒 PBCV-1 感染类小球藻：转录研究。
DOI：
10.1016/0042-6822(86)90413-7
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
VanEtten,JL
中科院分区：
文献类型：
--
作者：
Schuster,AM;Girton,L;Burbank,DE;VanEtten,JL
单细胞真核小球藻样绿色菌NC 64 A被含有大双链DNA的病毒PBCV-1感染后，立即减少宿主RNA的合成。叶绿体rRNA，但不是胞质rRNA，病毒感染后降解。利用PBCV-1 DNA的四个克隆片段作为探针的北方印迹分析，其代表约12%的病毒基因组，揭示了PBCV-1转录的几个特性：（i）在感染后5分钟内检测到少量病毒转录物。(ii)每个PBCV-1 DNA克隆与早期和晚期转录物杂交，这意味着早期和晚期基因分散在整个病毒基因组中。(iii)从早期转录到晚期转录的转变发生在感染后40至60分钟之间，与病毒DNA合成的开始一致。(iv)四个DNA克隆中有三个与转录本杂交，其加性大于相应的DNA探针。这可能反映了RNA加工、重叠基因的存在或两条DNA链的转录。(v)一些，但不是所有的，早期的成绩单在放线菌酮的存在下合成。这表明病毒携带自身的RNA聚合酶或使用宿主RNA聚合酶进行非常早期的病毒转录，并且额外的后期转录物的合成取决于早期基因产物的翻译。
Infection of the unicellular, eukaryoticChlorella-like green alga NC64A by the large dsDNA containing virus PBCV-1 immediately reduced host RNA synthesis. Chloroplast rRNAs, but not cytosolic rRNAs, were degraded following viral infection. Northern blot analysis utilizing four cloned fragments of PBCV-1 DNA as probes, which represent about 12% of the viral genome, revealed several properties of PBCV-1 transcription: (i) A few viral transcripts were detected within 5 min after infection. (ii) Each PBCV-1 DNA clone hybridized to both early and late transcripts which implies that early and late genes are dispersed throughout the viral genome. (iii) The transition from early to late transcription occurred between 40 and 60 min after infection coincident with the onset of viral DNA synthesis. (iv) Three of the four DNA clones hybridized to transcripts which additively were larger than the corresponding DNA probe. This could reflect RNA processing, presence of overlapping genes, or transcription from both DNA strands. (v) A few, but not all, early transcripts were synthesized in the presence of cycloheximide. This suggests that the virus either carries in its own RNA polymerase or uses a host RNA polymerase for very early viral transcription and that synthesis of additional, later transcripts depends on translation of an early gene product(s).